CAJ | 학술논문

研究了Erwinia persicinus RA2和Bacillus pumilus WP8浸种和拌土处理对番茄青枯病的实际防治效果,及其对番茄根际微生物群落的影响。结果显示,两株菌都具有防治番茄青枯病的作用,并能不同程度地促进番茄幼苗生长。主要体现在显著提高番茄幼苗健株率,病原菌处理的健株率最低,仅为22.4%,而RA2和WP8浸种处理分别达68.9%和62.8%;促进幼苗地上部增高、增粗和根部生长,如WP8浸种处理的茎叶干重和根干重分别达到4.87 mg·株^-1和35.69 mg·株^-1,分别比病原菌对照提高110.82%和205.83%。浸种处理的促进效应明显优于拌土处理;还能在一定程度上提高土壤水稳性团聚体（〉0.25 mm）比例,WP8浸种处理尤为明显,分别比空白对照和病原菌对照提高269.91%和156.88%。DGGE指纹图谱表明根际微生物群落受番茄种植的影响最大,其次是青枯病菌,而受这两种菌施用的影响最小。
연구료Erwinia persicinus RA2화Bacillus pumilus WP8침충화반토처리대번가청고병적실제방치효과,급기대번가근제미생물군락적영향。결과현시,량주균도구유방치번가청고병적작용,병능불동정도지촉진번가유묘생장。주요체현재현저제고번가유묘건주솔,병원균처리적건주솔최저,부위22.4%,이RA2화WP8침충처리분별체68.9%화62.8%;촉진유묘지상부증고、증조화근부생장,여WP8침충처리적경협간중화근간중분별체도4.87 mg·주^-1화35.69 mg·주^-1,분별비병원균대조제고110.82%화205.83%。침충처리적촉진효응명현우우반토처리;환능재일정정도상제고토양수은성단취체（〉0.25 mm）비례,WP8침충처리우위명현,분별비공백대조화병원균대조제고269.91%화156.88%。DGGE지문도보표명근제미생물군락수번가충식적영향최대,기차시청고병균,이수저량충균시용적영향최소。
The effect of Erwinia persicinus RA2 and Bacillus pumilus WP8 with soaking and mixing soil treatments on tomato bacterial wilt and tomato rhizosphere bacterial community were studied.The results showed that both E.persicinus RA2 and B.pumilus WP8 could effectively control tomato bacterial wilt,and promote growth of tomato seedlings.Inoculation method with the seeds soaking was more effective than soil drench.The rates of healthy stand treated by seeds soaking were 68.9% and 62.8% respectively,significantly higher when compared to control and Ralstonia solanacearum QL-Rs1115 treatment;The dry biomass of stem and leaf and root reached 4.87 mg·strain^-1 and 35.69 mg·strain^-1 in B.pumilus WP8 soaking treatment,which were improved by 110.82% and 205.83% compared to R.solanacearum QL-Rs1115 treatment;Also,soil aggregate stability（〉0.25 mm） ratio were increased 269.91% and 156.88% compared to control and R.solanacearum QL-Rs1115 treatment respectively.DGGE fingerprinting indicated that the uses of E.persicinus RA2 and B.pumilus WP8 had minimal effects on rhizosphere bacterial community.