CAJ | 학술논문

本文研究了生防促生芽孢杆菌OKB105、FZB42和以其为宿主菌的可外泌表达Harpin蛋白激发子的基因工程菌OKBHF、FZBHarpin对水稻白叶枯病的防治效果。菌株OKB105、FZB42、OKBHF和FZBHarpin温室防效分别为31.7%、23.6%、43.2%和56.7%;田间防效分别为29.8%、21.8%、40.4%和43.1%,表达Harpin蛋白的芽孢杆菌工程菌的防病效果显著高于出发菌株。两株生防芽孢杆菌及其工程菌对水稻均有良好的促生作用,特别是对水稻植株的生长促进明显,其中FZBHarpin使植株增高达15.86%。田间产量统计结果显示,OKBHF增产效果最显著,为14%,产量达8869 kg·hm^-2。工程菌比出发菌株显著提高水稻抗病性和促进植物生长,在农业生产上具有应用的潜力。
본문연구료생방촉생아포간균OKB105、FZB42화이기위숙주균적가외비표체Harpin단백격발자적기인공정균OKBHF、FZBHarpin대수도백협고병적방치효과。균주OKB105、FZB42、OKBHF화FZBHarpin온실방효분별위31.7%、23.6%、43.2%화56.7%;전간방효분별위29.8%、21.8%、40.4%화43.1%,표체Harpin단백적아포간균공정균적방병효과현저고우출발균주。량주생방아포간균급기공정균대수도균유량호적촉생작용,특별시대수도식주적생장촉진명현,기중FZBHarpin사식주증고체15.86%。전간산량통계결과현시,OKBHF증산효과최현저,위14%,산량체8869 kg·hm^-2。공정균비출발균주현저제고수도항병성화촉진식물생장,재농업생산상구유응용적잠력。
In this study,Bacillus OKB105、FZB42 and their genetically engineered bacteria OKBHF and FZBHarpin,which express and secret Harpin proteins,were determined for their biocontrol efficacy on rice bacterial leaf blight.Tests indicated that,control efficiacies of the four strains OKB105,FZB42,OKBHF and FZBHarpin were 31.7%,23.6%,43.2% and 56.7%,respectively,in greenhouse,while 29.8%,21.8%,40.4% and 43.1%,respectively,in field.Compared with patent strains,genetically engineered bacteria showed higher biocontrol efficacy and more significant plant growth-promoting capability,especially FZBHarpin which increased rice height by 15.86%.Results also indicated that yield of rice treated with OKBHF reached up to 8869 kg ·ha^- 1 by 14% increment.