中国生物防治学报
中國生物防治學報
중국생물방치학보
CHINESE JOURNAL OF BIOLOGICAL CONTROL
2012年
2期
293-297
,共5页
蔡守平%何学友%曾丽琼%黄金水%钟景辉%嵇保中
蔡守平%何學友%曾麗瓊%黃金水%鐘景輝%嵇保中
채수평%하학우%증려경%황금수%종경휘%혜보중
金龟子绿僵菌%星天牛%产孢量%致病力%致死中时(LT50)%僵虫率
金龜子綠僵菌%星天牛%產孢量%緻病力%緻死中時(LT50)%僵蟲率
금구자록강균%성천우%산포량%치병력%치사중시(LT50)%강충솔
Metarhizium anisopliae%Anoplophora chinensis%sporulation%virulence%median lethal time(LT50)%cadaver rate
室内测定了6株金龟子绿僵菌菌株的菌落生长速率、产孢量及其对星天牛幼虫的致病力。结果表明：不同菌株生长速率和产孢量存在显著差异,在PPDA培养基上,菌株MaYTTR04和MaZPTR01的菌落生长较快,培养15 d后,菌落直径分别为6.3 cm和5.9 cm;产孢量以菌株MaYTTR04最大,MaZPTR01其次,分别为2.0×10^8孢子·cm^-2和1.1×10^8孢子·cm^-2,显著高于其他菌株。生物测定结果表明,不同绿僵菌菌株对星天牛幼虫的致病力差异显著,接种20 d后,星天牛幼虫累积死亡率在40%～96.7%之间;菌株MaZPTR01和MaYTTR04对星天牛幼虫致死率分别达到96.7%和93.3%;僵虫率也高,分别达到86.7%和80%;致死中时（LT50）短,分别为5.71 d和5.80 d。研究结果表明,菌株MaZPTR01和MaYTTR04对星天牛幼虫致病力较强,同时具备良好的生产性能,故可作为优良菌株用于星天牛的防治。
室內測定瞭6株金龜子綠僵菌菌株的菌落生長速率、產孢量及其對星天牛幼蟲的緻病力。結果錶明：不同菌株生長速率和產孢量存在顯著差異,在PPDA培養基上,菌株MaYTTR04和MaZPTR01的菌落生長較快,培養15 d後,菌落直徑分彆為6.3 cm和5.9 cm;產孢量以菌株MaYTTR04最大,MaZPTR01其次,分彆為2.0×10^8孢子·cm^-2和1.1×10^8孢子·cm^-2,顯著高于其他菌株。生物測定結果錶明,不同綠僵菌菌株對星天牛幼蟲的緻病力差異顯著,接種20 d後,星天牛幼蟲纍積死亡率在40%～96.7%之間;菌株MaZPTR01和MaYTTR04對星天牛幼蟲緻死率分彆達到96.7%和93.3%;僵蟲率也高,分彆達到86.7%和80%;緻死中時（LT50）短,分彆為5.71 d和5.80 d。研究結果錶明,菌株MaZPTR01和MaYTTR04對星天牛幼蟲緻病力較彊,同時具備良好的生產性能,故可作為優良菌株用于星天牛的防治。
실내측정료6주금구자록강균균주적균락생장속솔、산포량급기대성천우유충적치병력。결과표명：불동균주생장속솔화산포량존재현저차이,재PPDA배양기상,균주MaYTTR04화MaZPTR01적균락생장교쾌,배양15 d후,균락직경분별위6.3 cm화5.9 cm;산포량이균주MaYTTR04최대,MaZPTR01기차,분별위2.0×10^8포자·cm^-2화1.1×10^8포자·cm^-2,현저고우기타균주。생물측정결과표명,불동록강균균주대성천우유충적치병력차이현저,접충20 d후,성천우유충루적사망솔재40%～96.7%지간;균주MaZPTR01화MaYTTR04대성천우유충치사솔분별체도96.7%화93.3%;강충솔야고,분별체도86.7%화80%;치사중시（LT50）단,분별위5.71 d화5.80 d。연구결과표명,균주MaZPTR01화MaYTTR04대성천우유충치병력교강,동시구비량호적생산성능,고가작위우량균주용우성천우적방치。
Colony growth rates and sporulation of 6 strains of Metarhizium anisopliae and their virulence to Anoplophora chinensis larvae were investigated in laboratory.Results showed that colony growth rates and sporulation of different strains were significantly different.Colony growth rates of the strains MaYTTR04 and MaZPTR01 were relatively higher than those of other strains,with colony diameters of 6.3 cm and 5.9 cm on PPDA plate at 15 days after inoculation,respectively.Sporulation of these two strains was 2.0×10^8 spores ·cm ^-2 and 1.1×10^8 spores· cm^- 2,significantly higher than that of other strains.Bioassay results showed that virulence of different M.anisopliae strains against to A.chinensis larvae was significantly different,with mortalities ranged from 40% to 96.7% at 20 days after inoculation.Mortalities of the larvae inoculated with MaZPTR01and MaYTTR04 were 96.7% and 93.3%,respectively,much higher than those inoculated with other strains.The cadaver rates of larvae inoculated with these two strains were 86.7% and 80%,respectively,significantly higher than those of other strains.The median lethal time（LT50） of MaZPTR01and MaYTTR04 was 5.71 d and 5.80 d,respectively.The results showed that the two M.anisopliae strains,MaZPTR01and MaYTTR04,had high virulence against to A.chinensis larvae,and also were excellent at growth performance.Therefore,these two strains can be the potential candidate in A.chinensis control.
