CAJ | 학술논문

药用植物内生真菌是一类重要的微生物资源,能代谢产生多种生物活性物质,本研究从浙江天目山自然保护区银杏Ginkgo biloba中分离获得1株活性菌株TMSF169。通过形态学和ITS rDNA序列分析,鉴定该菌株为团青霉Penicillium commune。发酵粗提物采用正相硅胶柱层析和Sephadex LH-20凝胶柱层析,以紫外光或碘蒸汽显迹,配合活性追踪,从菌株TMSF169发酵液中分离获得1个具抗真菌及除草活性的化合物TMSF169A,通过质谱和核磁共振波谱等技术将其结构鉴定为圆弧菌醛酸（cyclopaldic acid）。活性测试表明,该化合物具有抗菌活性,对鸭跖草Commelina communis,反枝苋Amaranthus retroflexus,马唐Digitaria sanguinalis、空心莲子草Alternanthera philoxeroides、水稻Oryza sativa等植物均具有致病毒性,对芝麻Sesamum indicum和大豆Glycine max不敏感。结果显示菌株TMSF169具有较好的开发应用价值。
약용식물내생진균시일류중요적미생물자원,능대사산생다충생물활성물질,본연구종절강천목산자연보호구은행Ginkgo biloba중분리획득1주활성균주TMSF169。통과형태학화ITS rDNA서렬분석,감정해균주위단청매Penicillium commune。발효조제물채용정상규효주층석화Sephadex LH-20응효주층석,이자외광혹전증기현적,배합활성추종,종균주TMSF169발효액중분리획득1개구항진균급제초활성적화합물TMSF169A,통과질보화핵자공진파보등기술장기결구감정위원호균철산（cyclopaldic acid）。활성측시표명,해화합물구유항균활성,대압척초Commelina communis,반지현Amaranthus retroflexus,마당Digitaria sanguinalis、공심련자초Alternanthera philoxeroides、수도Oryza sativa등식물균구유치병독성,대지마Sesamum indicum화대두Glycine max불민감。결과현시균주TMSF169구유교호적개발응용개치。
Endophytic fungi in medicinal plants are important sources for microorganisms which can produce a variety of bioactive compounds.In this study,an endophytic fungus TMSF169 with antifungal activity was isolated from Ginkgo biloba in Tianmushan Nature Reserve,Zhejiang province.TMSF169 was identified as Penicillium commune based on morphology and ITS rDNA sequence analysis.An antifungal and herbicidal compound TMSF169A was isolated and purified from the fermentation broth of TMSF169 through normal-phase silica gel column chromatography and Sephadex LH-20 gel column chromatography,traced by ultraviolet light or iodine vapor with bioassay-guided fractionation.TMSF169A was elucidated as cyclopaldic acid based on mass spectrometry and nuclear magnetic resonance spectroscopy.Antifungal activity assays showed that the compound had inhibitory to a series of plant pathogenic fungi.The metabolite was also pathogenic to Commelina communis,Digitaria sanguinalis,Amaranthus retroflexus,Alternanthera philoxeroides and Oryza sativa,but not sensitive to Sesamum indicum and Glycine max.All the results demonstrated that the strain TMSF169 deserves to develop further.