CAJ | 학술논문

禾长蠕孢菌稗草专化型（Helminthosporium gramineum Rabenh. f. sp. echinochloae,HGE）是一株稗草生防潜力菌,为了获得该生防菌大量孢子用于田间试验,本文研究了其固体发酵产孢最佳培养基配方及其培养条件。通过试验筛选确定了固体发酵培养基所需碳源及其浓度、氮源及其浓度以及底物基质,适合HGE菌孢子生产的培养基最佳组合为：以珍珠岩为底物基质,在其中添加4%米粉、1%豆粕粉、0.2%Na3PO4.12H2O、0.1%MgSO4.7H2O。同时确定了适合HGE菌孢子生产的培养条件：培养基最适含水量为40%,最佳接菌量为8%菌悬液,25℃静置培养11 d,培养期间用黑光灯12 h循环光照。按优化后培养条件放大培养HGE菌,获得最高产孢量可以达到1.5×107孢子·克-1干物质。温室生测结果显示优化条件生产的HGE菌孢子对稗草防效可达80%以上。
화장연포균패초전화형（Helminthosporium gramineum Rabenh. f. sp. echinochloae,HGE）시일주패초생방잠력균,위료획득해생방균대량포자용우전간시험,본문연구료기고체발효산포최가배양기배방급기배양조건。통과시험사선학정료고체발효배양기소수탄원급기농도、담원급기농도이급저물기질,괄합HGE균포자생산적배양기최가조합위：이진주암위저물기질,재기중첨가4%미분、1%두박분、0.2%Na3PO4.12H2O、0.1%MgSO4.7H2O。동시학정료괄합HGE균포자생산적배양조건：배양기최괄함수량위40%,최가접균량위8%균현액,25℃정치배양11 d,배양기간용흑광등12 h순배광조。안우화후배양조건방대배양HGE균,획득최고산포량가이체도1.5×107포자·극-1간물질。온실생측결과현시우화조건생산적HGE균포자대패초방효가체80%이상。
Helminthosporium gramineum Rabenh f. sp. echinochloae （HGE） is a potential biocontrol agent for bamyardgrass control in rice field. Authors optimized the medium and culture conditions in a solid state fermentation system for mass production ofHGE conidia. Investigation on carbon source, nitrogen source and solid substrate indicated that a medium consisted of 4% rice flour, 1% soybean meal, 0.2% Na3PO4·12H2O, 0.1% MgSO4·7H2O and pedite was better for high yield of condia. 40% medium moisture, 8% inoculum concentration, 25℃ and 11 d incubation were good for the conidial production. By the medium and culture conditions, the maximum conidial yield was 1.5×107 conidia.g-1 dry material. Pot bioassay in the greenhouse gave rise to 80% control efficacy against barnyardgrass after treatment with HGE conidia at 0.6 g-m-2.