CAJ | 학술논문

以琼胶寡糖处理坛紫菜Porphyra haitanensis,激发其防御反应,坛紫菜在18℃生长和35℃热激时的病烂现象得到明显改善。寡糖激发的防御信号能够杀死附生在藻上的细菌,健康藻上约94.7%的附生菌群在加入100μg.mL-1琼胶寡糖1 h内被杀死,但过氧化氢酶可部分抑制这种作用。利用3种抗生素组合获得无菌坛紫菜体系,回染致病细菌后,约60.8%的回染细菌在寡糖激发后的3 h内被消灭。这种附生细菌的减少现象不是源于琼胶寡糖的直接抑菌作用,可能与琼胶寡糖引起坛紫菜H2O2的瞬时暴发有关。结果表明,琼胶寡糖能够作为坛紫菜的抗性激发子,通过诱导坛紫菜自身的防御反应来减少附生细菌的数量,从而保持藻体的健康生长。
이경효과당처리단자채Porphyra haitanensis,격발기방어반응,단자채재18℃생장화35℃열격시적병란현상득도명현개선。과당격발적방어신호능구살사부생재조상적세균,건강조상약94.7%적부생균군재가입100μg.mL-1경효과당1 h내피살사,단과양화경매가부분억제저충작용。이용3충항생소조합획득무균단자채체계,회염치병세균후,약60.8%적회염세균재과당격발후적3 h내피소멸。저충부생세균적감소현상불시원우경효과당적직접억균작용,가능여경효과당인기단자채H2O2적순시폭발유관。결과표명,경효과당능구작위단자채적항성격발자,통과유도단자채자신적방어반응래감소부생세균적수량,종이보지조체적건강생장。
The rot disease of Porphyra haitanens＆ cultured under 18℃ and 35℃ were obviously lightened by pretreatment with oligoagars. The defense response elicited by oligoagars was strong enough to kill epiphytic bacteria associated with the algal. Up to 94.7% of the resident bacterial flora of healthy plants was eliminated within 60 min after treatment by 100μg.mL-1 oligoagars. Addition of catalase prior to oligoagars partially inhibited this elimination. Mixture of three kinds of antibiotics was used to treat P haitanensis to get the asepsis culture systems. Approximately 60.8% of reinfected pathogenic bacteria on axenic algal were eliminated within 3 h after elicitation by oligoagars. Antimicrobial test revealed that oligoagars showed no direct toxicity to associated bacteria or pathogen, but a rapid release of hydrogen peroxide was observed after adding of oligoagars in medium. From these results, we infer that oligoagars can be recognized as defense elicitors by P. haitanensis to protect algal efficiently from infections by eliminating the associated pathogenic bacteria.