CAJ | 학술논문

蛋白酶抑制剂是一类能够抑制蛋白水解酶活性的小分子蛋白质，在昆虫肠道内通过抑制蛋白酶的活性，可以干扰昆虫的正常生长和发育。本文利用体外重组表达的蛋白酶抑制剂Xbpi-1，研究了Xbpi-1对豌豆蚜Acyrthosiphon pisum Harris的杀虫活性及其生化机制。结果表明，取食含蛋白酶抑制剂Xbpi-1浓度为50、200、400、800μg·mL^-1人工饲料的豌豆蚜，96h后的校正死亡率分别为2．1％、9-4％、19．7％和50．9％，虫重分别减少12_3％、20．3％、25．7％和33．7％。生化测定结果表明，4种浓度处理的豌豆蚜体内总蛋白酶、氨肽酶、胰蛋白酶、胰凝乳蛋白酶的活性均有显著降低，浓度为800μ·mL^-1时，其酶活性分别降低了29．6％、26．1％、23．5％和23．8％。本研究结果说明，蛋白酶抑制剂Xbpi-1对豌豆蚜具有较好的生物活性，为进一步开发应用于豌豆蚜等刺吸式害虫的防治提供了理论依据。
단백매억제제시일류능구억제단백수해매활성적소분자단백질，재곤충장도내통과억제단백매적활성，가이간우곤충적정상생장화발육。본문이용체외중조표체적단백매억제제Xbpi-1，연구료Xbpi-1대완두아Acyrthosiphon pisum Harris적살충활성급기생화궤제。결과표명，취식함단백매억제제Xbpi-1농도위50、200、400、800μg·mL^-1인공사료적완두아，96h후적교정사망솔분별위2．1％、9-4％、19．7％화50．9％，충중분별감소12_3％、20．3％、25．7％화33．7％。생화측정결과표명，4충농도처리적완두아체내총단백매、안태매、이단백매、이응유단백매적활성균유현저강저，농도위800μ·mL^-1시，기매활성분별강저료29．6％、26．1％、23．5％화23．8％。본연구결과설명，단백매억제제Xbpi-1대완두아구유교호적생물활성，위진일보개발응용우완두아등자흡식해충적방치제공료이론의거。
Protease inhibitors are capable of affecting insect survival and development by inhibition of the activities of proteases. In this study, the biological activity of recombinant protease inhibitor Xbpi-1 against the pea aphid, Acyrthosiphon pisum Harris, and the effects on the activities of aphid proteases were evaluated. The pea aphids fed on artificial diet containing 50, 200, 400, 800 μg·mL^-1 Xbpi-1 protein showed calibrated mortality of 2.1%, 9.4%, 19.7%, 50.9%, and aphid weights were reduced 12.3%, 20.3%, 25.7%, 33.7%, respectively compared with those of the control. The results showed that the activities of pea aphid proteases (trypsin, chymotrypsin, leucine aminopeptidase and total protinase) were significantly inhibited by the protease inhibitor protein. The results indicated that the protease inhibitor protein used in this study may be a potential bio-pesticide for controlling of sucking insect pests.