CAJ | 학술논문

皂苷被认为是植物抵抗病原真菌侵袭的防御物质,具有抗真菌活性.许多成功侵染植物的真菌通过产生皂苷解毒酶水解皂苷糖链上的糖残基,解除皂苷的毒性.本文研究了人参锈腐病菌毁灭柱孢菌Cylindrocarpon destructans和非人参病原菌构巢曲霉菌Aspergillus nidulans降解人参皂苷的能力.结果显示,毁灭柱孢菌能够产生胞外糖苷酶,水解二醇型人参皂苷Rb1、Rb2、Rc和Rd的C-3位和C-20位末端单糖,产生四种主要产物：绞股蓝皂苷XVII（G-XVII）、C-O、Mb和人参皂苷F2,而构巢曲霉菌只能将人参皂苷Rb1降解为Rd,不能降解其它的人参皂苷.这些结果说明,人参皂苷降解酶在毁灭柱孢菌的致病过程中可能发挥了重要作用.
조감피인위시식물저항병원진균침습적방어물질,구유항진균활성.허다성공침염식물적진균통과산생조감해독매수해조감당련상적당잔기,해제조감적독성.본문연구료인삼수부병균훼멸주포균Cylindrocarpon destructans화비인삼병원균구소곡매균Aspergillus nidulans강해인삼조감적능력.결과현시,훼멸주포균능구산생포외당감매,수해이순형인삼조감Rb1、Rb2、Rc화Rd적C-3위화C-20위말단단당,산생사충주요산물：교고람조감XVII（G-XVII）、C-O、Mb화인삼조감F2,이구소곡매균지능장인삼조감Rb1강해위Rd,불능강해기타적인삼조감.저사결과설명,인삼조감강해매재훼멸주포균적치병과정중가능발휘료중요작용.
Saponins is of antifungal properties and are considered to be protective agents against pathogens. A number of fungi invading successfully plants containing saponins produce saponin-detoxifying enzymes which could remove sugar residues from the saponin to relieve the toxicity of saponins. Ginseng rust rot pathogen Cylindrocarpon destructans and non-pathogenic fungus Aspergillus nidulans were examined for their abilities to degrade ginsenosides. Results showed that C. destructans degraded 20(S)-protopanaxadiol ginsenosides by an extracellular glycosidase through consecutive hydrolysis of terminal monosaccharide units from the sugar chains attached at C-3 and C-20 of ginsenosides Rbl, Rb2, Rc and Rd to yield four major products, gypenoside XVII (G-XVII), compound O, ginsenoside Mb, and ginsenoside F2. A. nidulans degraded ginsenoside Rbl into Rd as the final product, and did not transform the other ginsenosides. These results indicated that saponin-detoxifying enzyme was an important factor in determining pathogenicity of C. destructans to attack ginseng.