CAJ | 학술논문

　　为了提高小麦花药单倍体植株的加倍效率，以小麦冀5006/9204和核生2号/08004 F1杂交组合为试材，用不同浓度的秋水仙碱分别对小麦花药和植株分蘖节进行了处理，其中花药处理方法为在 C17诱导培养基中分别加入浓度0.02‰、0.05‰和0.10‰的秋水仙碱，分蘖节处理方法为分别用浓度0.20‰、0.50‰、0.75‰和1.00‰的秋水仙碱浸泡单倍体再生植株分蘖节各5 h和10 h，研究秋水仙碱不同浓度与处理方法对染色体加倍率的影响。结果表明：在含有秋水仙碱0.02‰的诱导培养基上再生单倍体植株的染色体加倍率最高，冀5006/9204和核生2号/08004 F1代杂交组合的染色体加倍效率分别为52.38％和47.37％；0.75‰秋水仙碱溶液浸泡分蘖节5 h的染色体加倍率最高，为65.06％。尽管我们用秋水仙碱处理花药的加倍率不如处理分蘖节的效果好，但该方法可能比处理分蘖节更经济、简便，可达到快繁与加倍同时进行的目的。
　　위료제고소맥화약단배체식주적가배효솔，이소맥기5006/9204화핵생2호/08004 F1잡교조합위시재，용불동농도적추수선감분별대소맥화약화식주분얼절진행료처리，기중화약처리방법위재 C17유도배양기중분별가입농도0.02‰、0.05‰화0.10‰적추수선감，분얼절처리방법위분별용농도0.20‰、0.50‰、0.75‰화1.00‰적추수선감침포단배체재생식주분얼절각5 h화10 h，연구추수선감불동농도여처리방법대염색체가배솔적영향。결과표명：재함유추수선감0.02‰적유도배양기상재생단배체식주적염색체가배솔최고，기5006/9204화핵생2호/08004 F1대잡교조합적염색체가배효솔분별위52.38％화47.37％；0.75‰추수선감용액침포분얼절5 h적염색체가배솔최고，위65.06％。진관아문용추수선감처리화약적가배솔불여처리분얼절적효과호，단해방법가능비처리분얼절경경제、간편，가체도쾌번여가배동시진행적목적。
For the purpose of improving the double efficiency of haploid plant in wheat tissue culture , F1 hybrid combinations of Ji 5006/9204 and Hesheng No.2/08004 were used, anther and the tillering nodes of the haploid plants were treated by different concentrations of colchicine , respectively.The anthers processing method was added colchicine with three concentrations of 0.02‰, 0.05‰ and 0.10‰ to the culture medium induced by C17.Whereas tillering node of the haploid plants was dipped in 0.20‰, 0.50‰, 0.75‰ and 1.00‰ colchicine at 5 h and 10 h, respectively.The effects of different concentrations of colchicine and processing methods on chromosome doubling rate were studied.The results indicated that the chromosome doubling rate in the culture medium induced by C17 which added 0.02‰ colchicine was highest, and the chromosome doubling efficiency of F1 hybrid combinations of Ji 5006/9204 and Hesheng No.2/08004 were 52.38% and 47.37% respectively.The tillering node was dipped in 0.75‰ colchicine for 5 h with the highest chromosome doubling efficiency , the doubling rate was 65.05%.Although the doubling frequency of anther treatment with colchicine was lower than tillering node treatment, it was less expensive and more convenient.Therefore, it was appropriate method for haploid breeding in the future.