CAJ | 학술논문

　　目的利用胶体金免疫层析技术对因药物滥用致使尿液中产生的四氢大麻酚酸检测试纸条进行研制。方法采用柠檬酸三钠法还原氯金酸制备胶体金。结果确立了制备四氢大麻酚酸检测试纸条的最佳条件：标记10μg/mL的抗四氢大麻酚酸单克隆抗体,将金标抗体按喷量2.0μL/cm喷涂在已处理的聚酯膜上,最后将四氢大麻酚酸-牛血清白蛋白结合物和羊抗鼠按0.5、1.0 mg/mL包被在硝酸纤维素膜(NC膜)上,经过37℃烘箱干燥16 h,组装制备四氢大麻酚酸检测试纸条。结论这种方法能在3~5 min内快速检测出尿液中残留的最小浓度为50 ng/mL的四氢大麻酚酸。
　　목적이용효체금면역층석기술대인약물람용치사뇨액중산생적사경대마분산검측시지조진행연제。방법채용저몽산삼납법환원록금산제비효체금。결과학립료제비사경대마분산검측시지조적최가조건：표기10μg/mL적항사경대마분산단극륭항체,장금표항체안분량2.0μL/cm분도재이처리적취지막상,최후장사경대마분산-우혈청백단백결합물화양항서안0.5、1.0 mg/mL포피재초산섬유소막(NC막)상,경과37℃홍상간조16 h,조장제비사경대마분산검측시지조。결론저충방법능재3~5 min내쾌속검측출뇨액중잔류적최소농도위50 ng/mL적사경대마분산。
Objective To develop a tetrahydrocannabinol (THC) acid test strip by colloidal gold immu-nochromatographic technique. Methods Colloidal gold was prepared through reduction of chloroauric acid by trisodium citrate. Results The optimal conditions for preparing the THC acid test strip were as follows:10 μg/mL anti-THC acid monoclonal antibody was labeled, then the gold-labeled antibody was sprayed on a treated polyester film at a volume of 2.0 μL/cm, finally 0.5 mg/mL tetrahydrocannabinol acid-BSA combina-tion and 1.0 mg/mL goat anti-mouse antibody were used to coat the nitrocellulose (NC) membrane. After oven drying at 37 ℃ for 16 h, the tetrahydrocannabinol acid detection reagent was assembled. Conclusion The established method can rapidly detect residual THC acid at a minimum concentration of 50 ng/mL in urine in 3~5 min.