CAJ | 학술논문

　　目的对不同重金属胁迫蔬菜产生金属硫蛋白(金属硫组氨酸三甲基内盐)进行同源性检测。方法用Cd-MT 免疫家兔产生 IgG 后，用不同浓度硫酸铜、乙酸铅、氯化钴、氯化汞等重金属胁迫萝卜幼苗、油白菜和油麦菜诱导产生金属硫蛋白，通过免疫印迹检测其同源性。结果 Cd-MT 与重金属胁迫三种蔬菜所产生的金属硫蛋白具有同源性；不同浓度的重金属对幼苗中 MTs表达量有影响。结论 Cd-MT诱导家兔产生的IgG可用于农产品中重金属污染的检测。
　　목적대불동중금속협박소채산생금속류단백(금속류조안산삼갑기내염)진행동원성검측。방법용Cd-MT 면역가토산생 IgG 후，용불동농도류산동、을산연、록화고、록화홍등중금속협박라복유묘、유백채화유맥채유도산생금속류단백，통과면역인적검측기동원성。결과 Cd-MT 여중금속협박삼충소채소산생적금속류단백구유동원성；불동농도적중금속대유묘중 MTs표체량유영향。결론 Cd-MT유도가토산생적IgG가용우농산품중중금속오염적검측。
Objective To develop the homologous detection method of metallothionein in vegetables un-der different heavy metal stress. Methods IgG were collected from Cd-MT immunized rabbit, and metallo-thionein expression in radish seedlings, pakchoi and leaf-used lettuce were introduced by adding different con-centration of CuSO4, lead acetate, CoCl2 and HgCl2 respectively. Immunoblotting technique was used for ho-mologous detection. Results Metallothionein synthesized in three kinds of vegetables under the stress of Cd-MT and heavy metal were homologous;the concentration of heavy metal influenced the expression of MTs in seeding. Conclusion The IgG collected from Cd-MT immunized rabbit can be used for detection of heavy metal contaminations in agricultural products.