CAJ | 학술논문

　　目的用衍生化法测定山药中还原型谷胱甘肽(GSH)和总巯基(-SH)的含量。方法利用4,4′-二硫代联吡啶(4-DPS)和邻苯二甲醛(OPA)为衍生化试剂,采用细胞破壁和衍生化为一体的样品前处理方法,分别用UV-vis分光光度法和荧光光谱法测定了山药鲜品中总巯基(-SH)、GSH的含量。结果山药中含较丰富的总巯基和 GSH,含量分别达0.4556μmol/g和0.1792μmol/g。UV-vis分光光度法和荧光光谱法测定方法的检测限分别为1.0458μmol/L和0.5666μmol/L,回收率分别为98.73%和98.40%。结论本研究对山药中巯基化合物的含量、测定方法学和样品前处理方法提供了重要的基础数据和参考依据。
　　목적용연생화법측정산약중환원형곡광감태(GSH)화총구기(-SH)적함량。방법이용4,4′-이류대련필정(4-DPS)화린분이갑철(OPA)위연생화시제,채용세포파벽화연생화위일체적양품전처리방법,분별용UV-vis분광광도법화형광광보법측정료산약선품중총구기(-SH)、GSH적함량。결과산약중함교봉부적총구기화 GSH,함량분별체0.4556μmol/g화0.1792μmol/g。UV-vis분광광도법화형광광보법측정방법적검측한분별위1.0458μmol/L화0.5666μmol/L,회수솔분별위98.73%화98.40%。결론본연구대산약중구기화합물적함량、측정방법학화양품전처리방법제공료중요적기출수거화삼고의거。
Objective To detect reduced glutathione (GSH) and total thiols (-SH) in Rhizoma Dioscoreae by derivatization methods. Methods Total thiols and reduced glutathione in Rhizoma Dioscoreae were de-termined by UV-vis spectrophotometry and fluorophotometry using 4,4′-dithiodipyridine (4-DPS) and o-phthaldehyde (OPA) as derivatization agents. Samples were prepared by simultaneous derivatization during cell rupture, Results 0.4556 μmol/g of total thiols and 0.1792 μmol/g of GSH were detected in the sample . The detection limits for UV-vis spectrophotometry and fluorophotometry were 1.0458 μmol/L and 0.5666 μmol/L, and recoveries were 98.73%and 98.40%, respectively. Conclusion This study provides useful data and references for the content of thiol compounds in Rhizoma Dioscore determination methodology and pre-treatment methods for plant sample analysis.