CAJ | 학술논문

　　目的建立同时检测4种食源性肠道致病菌的多重 PCR方法。方法分别依据沙门氏菌(Salmonella spp)的fimA基因、弗氏枸橼酸杆菌(Citrobacter freundii)的ldh基因、奇异变形杆菌(Proteus mirabilis)的idsC基因、迟缓爱德华菌(Edwardsiella tarda)的fimA基因设计4对特异性引物，并对多重PCR反应条件进行优化。结果建立的多重PCR方法具有结果可靠、灵敏度高、方便快捷的优点。结论本研究为研发快速检测以上4种食源性肠道致病菌的试剂盒提供了重要技术保障。
　　목적건립동시검측4충식원성장도치병균적다중 PCR방법。방법분별의거사문씨균(Salmonella spp)적fimA기인、불씨구연산간균(Citrobacter freundii)적ldh기인、기이변형간균(Proteus mirabilis)적idsC기인、지완애덕화균(Edwardsiella tarda)적fimA기인설계4대특이성인물，병대다중PCR반응조건진행우화。결과건립적다중PCR방법구유결과가고、령민도고、방편쾌첩적우점。결론본연구위연발쾌속검측이상4충식원성장도치병균적시제합제공료중요기술보장。
Objective To establish a method for detection of four species of food-borne pathogens syn-chronously by multiplex PCR. Methods Four pairs of suitable primers were designed according to the spe-cific gene fimA of Salmonella spp, gene ldh of Citrobacter freundii , gene idsC of Proteus mirabilis, and gene fimA of Edwardsiella tarda. The reaction conditions of multiplex PCR were optimized as well. Results The detection for real samples proved that the multiplex PCR method had the advantages of reliability, sensitivity and rapidness. Conclusion This method can provide a key technical support for developing a kit that detects the four species of food-borne pathogens rapidly.