CAJ | 학술논문

　　目的建立纸制食品接触材料中24种芳香族伯胺迁移量的液相色谱串联质谱检测方法。方法以不同种类食品模拟物进行模拟迁移实验,模拟迁移液过0.22μm滤膜测定。液相色谱使用ZORBAX-C18反相色谱柱,以乙腈和0.1%甲酸为流动相,在梯度条件下分析。在正离子模式下多反应监测(MRM)采集质谱信号,进行定性和定量分析。结果24种初级芳香胺在50%乙醇溶液(v/v)模拟物中标准曲线的线性相关系数为0.9902~0.9999,加标回收率为72.98%~97.88%,相对标准偏差均在0.30%~9.90%,定量限均小于或等于3.0μg/kg。结论该方法快速简便、灵敏度高、准确性好,满足了相关法规的限量测定要求。
　　목적건립지제식품접촉재료중24충방향족백알천이량적액상색보천련질보검측방법。방법이불동충류식품모의물진행모의천이실험,모의천이액과0.22μm려막측정。액상색보사용ZORBAX-C18반상색보주,이을정화0.1%갑산위류동상,재제도조건하분석。재정리자모식하다반응감측(MRM)채집질보신호,진행정성화정량분석。결과24충초급방향알재50%을순용액(v/v)모의물중표준곡선적선성상관계수위0.9902~0.9999,가표회수솔위72.98%~97.88%,상대표준편차균재0.30%~9.90%,정량한균소우혹등우3.0μg/kg。결론해방법쾌속간편、령민도고、준학성호,만족료상관법규적한량측정요구。
Objective To develop a liquid chromatography-tandem mass spectrometry (LC-MS-MS) method for the simultaneous determination of 24 primary aromatic amines (PAAs) released from food paper packaging mate-rials of food simulants. Methods Different food simulants were used to simulate the migration of PAAs from food paper packaging materials. The simulant migration liquid was determined directly after being filtered with a 0.22μm membrane without any other process. The analysis of PAAs was performed on a ZORBAX-C18 column using gra-dient elution with the mobile phase of 0.1%formic acid in water and acetonitrile, and the mass spectrometry signal was collected by the positive electrospray ionization-MS/MS method under MRM mode for qualitative and quantita-tive analyses. Results The correlation coefficients (r) were 0.9902~0.9999 for the standard curve of 24 primary aromatic amines in the simulant of 50%ethanol solution (v/v), recoveries ranged from 70.30%to 97.88%, the relative standard deviations were 0.30%~9.90%and the limit of quantification (LOQ) was 3.0μg/kg or lower. Conclusion This method is simple, fast, sensitive and accurate, and can meet the requirement of the relevant limit regulations.