CAJ | 학술논문

　　DNA条形码技术指利用一段或几段标准DNA序列实现动物、植物和真菌等物种的快速鉴定，是基于分子生物学进行物种分类研究的重要工具。近年，DNA条形码技术在中药鉴定中的应用日益增多，使中药质量评价研究进入一个崭新的时代。本文结合国内外文献，综述了DNA条形码技术在中药材鉴定、中药饮片和中成药质量评价方面的应用，以及DNA条形码技术鉴定物种流程。利用DNA条形码技术进行中药质量评价不受物种形态特征和发育时期的影响，且快速、准确，然而此技术不能从化学成分层面评价中药。将传统评价方法和DNA条形码技术相结合对中药进行全面质量评价，对推进我国中医药现代化的发展具有深远的意义。
　　DNA조형마기술지이용일단혹궤단표준DNA서렬실현동물、식물화진균등물충적쾌속감정，시기우분자생물학진행물충분류연구적중요공구。근년，DNA조형마기술재중약감정중적응용일익증다，사중약질량평개연구진입일개참신적시대。본문결합국내외문헌，종술료DNA조형마기술재중약재감정、중약음편화중성약질량평개방면적응용，이급DNA조형마기술감정물충류정。이용DNA조형마기술진행중약질량평개불수물충형태특정화발육시기적영향，차쾌속、준학，연이차기술불능종화학성분층면평개중약。장전통평개방법화DNA조형마기술상결합대중약진행전면질량평개，대추진아국중의약현대화적발전구유심원적의의。
DNA barcoding is an important tool for taxonomic analysis based on molecular biology. It can be used for the rapid identification of species with one or more standard DNA fragments. Recently, DNA barcoding has been widely used to identify traditional Chinese medicine (TCM), which has boosted advancement of TCM quality evaluation. This paper reviews the application of DNA barcoding in the identification of traditional Chinese medicinal materials and in the evaluation of quality of TCM slices and formulated products, and summarizes the steps of DNA barcoding for species identification. DNA barcoding technology is not affected by morphological characteristics and developmental period of species, and is rapid and accurate. However, this technology can not evaluate the chemical constitution of TCM. Therefore, combination of traditional evaluation method and DNA barcoding technology to conduct a comprehensive quality evaluation of TCM would be of profound significance for the modernization of TCM.