CAJ | 학술논문

　　目的研究二十二碳六烯酸（DHA）联合5氟尿嘧啶（5-FU）对人肝癌细胞HepG2耐药相关蛋白ERK、JNK及p38的表达。方法 Western blot检测5-FU 5μg/mL，或5-FU 5μg/mL联合DHA 30μg/mL作用HepG2细胞48 h后ERK、JNK及p38蛋白表达。结果与单用5-FU组比较，5-FU联合DHA组对耐药相关蛋白的影响，p-ERK明显受到抑制，而p-JNK表达显著增加，p-p38表达无差别。结论 DHA对肝癌细胞耐药相关蛋白的调控可能通过抑制ERK、激活JNK信号通路来增强5-FU的细胞毒活动，发挥增敏化疗药物的作用。
　　목적연구이십이탄륙희산（DHA）연합5불뇨밀정（5-FU）대인간암세포HepG2내약상관단백ERK、JNK급p38적표체。방법 Western blot검측5-FU 5μg/mL，혹5-FU 5μg/mL연합DHA 30μg/mL작용HepG2세포48 h후ERK、JNK급p38단백표체。결과여단용5-FU조비교，5-FU연합DHA조대내약상관단백적영향，p-ERK명현수도억제，이p-JNK표체현저증가，p-p38표체무차별。결론 DHA대간암세포내약상관단백적조공가능통과억제ERK、격활JNK신호통로래증강5-FU적세포독활동，발휘증민화료약물적작용。
Objective To study the expression of drug-resistance associated protein, such as ERK, JUK and P38, in hepatocellular carcinoma HepG2 cells treated with docosahexaenoic acid and 5-fluorouracil. Methods The expression of JNK, ERK and p38 were analyzed in HepG2 treated with 5-FU or 5-FU in combination with DHA for 48 hours by western blot. Results When compared with the group only treated with 5-FU, samples from the group treated with 5-FU and DHA showed different expression level of drug-resistance associated-proteins. Down regulation of p-ERK, up regulation of p-JNK and no change of p-p38 could be found in the latter group. Conclusion With inhibiting the p-ERK and activating p-JNK, DHA could regulate hepatocellular carcinoma cells to significantly enhance the cytotoxic activity of 5-FU. So that it could play a role in the sensitization of chemotherapy drugs.