分子诊断与治疗杂志
分子診斷與治療雜誌
분자진단여치료잡지
JOURNAL OF MOLECULAR DIAGNOSIS AND THERAPY
2013年
4期
227-231
,共5页
陈江涛%刘配芬%钟德善%潘雪峰%杨辉%杨立业%陆志为%杨惠钿%林敏%Santiago-m Monte-Nguba%Juan Carlos Salas Ehapo%Urbano Monsuy Eyi
陳江濤%劉配芬%鐘德善%潘雪峰%楊輝%楊立業%陸誌為%楊惠鈿%林敏%Santiago-m Monte-Nguba%Juan Carlos Salas Ehapo%Urbano Monsuy Eyi
진강도%류배분%종덕선%반설봉%양휘%양립업%륙지위%양혜전%림민%Santiago-m Monte-Nguba%Juan Carlos Salas Ehapo%Urbano Monsuy Eyi
疟疾%荧光定量PCR%SYBR Green I%虫种鉴别
瘧疾%熒光定量PCR%SYBR Green I%蟲種鑒彆
학질%형광정량PCR%SYBR Green I%충충감별
Plasmodiumspp%Real-time quantitative PCR%SYBR Green I%Species identification
目的评价一种可以快速定量检测疟原虫及虫种鉴别的荧光定量PCR法。方法将SYBR Green I法与TaqMan探针法检测疟原虫进行比对，评价定量的准确性及方法的敏感度。以PCR-DNA测序法为金标准，评价SYBR Green I法分型的准确性。结果两个方法的定量拷贝检测数无显著性差异（P>0.05）。SYBR Green I法检测疟原虫的最低检测下限为1×102 copy/mL至5×102 copy/mL之间。与PCR-DNA测序法比较，SYBR Green I法能准确的对感染的疟原虫虫种做准确的鉴定。结论 SYBR Green I染料法定量可同时进行人体疟原虫的定量检测和虫种鉴别，且成本低廉，操作简便快速，可以在疟疾的高发地区推广使用。
目的評價一種可以快速定量檢測瘧原蟲及蟲種鑒彆的熒光定量PCR法。方法將SYBR Green I法與TaqMan探針法檢測瘧原蟲進行比對，評價定量的準確性及方法的敏感度。以PCR-DNA測序法為金標準，評價SYBR Green I法分型的準確性。結果兩箇方法的定量拷貝檢測數無顯著性差異（P>0.05）。SYBR Green I法檢測瘧原蟲的最低檢測下限為1×102 copy/mL至5×102 copy/mL之間。與PCR-DNA測序法比較，SYBR Green I法能準確的對感染的瘧原蟲蟲種做準確的鑒定。結論 SYBR Green I染料法定量可同時進行人體瘧原蟲的定量檢測和蟲種鑒彆，且成本低廉，操作簡便快速，可以在瘧疾的高髮地區推廣使用。
목적평개일충가이쾌속정량검측학원충급충충감별적형광정량PCR법。방법장SYBR Green I법여TaqMan탐침법검측학원충진행비대，평개정량적준학성급방법적민감도。이PCR-DNA측서법위금표준，평개SYBR Green I법분형적준학성。결과량개방법적정량고패검측수무현저성차이（P>0.05）。SYBR Green I법검측학원충적최저검측하한위1×102 copy/mL지5×102 copy/mL지간。여PCR-DNA측서법비교，SYBR Green I법능준학적대감염적학원충충충주준학적감정。결론 SYBR Green I염료법정량가동시진행인체학원충적정량검측화충충감별，차성본저렴，조작간편쾌속，가이재학질적고발지구추엄사용。
Objective To evaluate a SYBR Green I real-time PCR method for quantitative detection and species identification of human Plasmodium spp. Methods The SYBR Green I method was compared with TaqMan real-time PCR for investigation of the accuracy and sensitivity. At the same time, it was compared with the PCR-DNA sequencing for accuracy of the species identification. Results There were no significant difference between quantitative results of the SYBR Green I method and the TaqMan real-time PCR (P>0.05). The limit range of detection located from 1×102 copy/mL to 5×102 copy/mL. Compared with the PCR-DNA sequencing, it was accurate to identify the species of Plasmodiumspp. Conclusion The SYBR Green I real-time PCR method was lower cost, convenient and rapid for human Plasmodium spp. quantitative detection and species identification in the high prevalence area.
