CAJ | 학술논문

　　在东经122°21′，北纬39°34′大连黄海海域的底泥中分离得到一株海洋细菌，经系统的微生物学鉴定及16SrDNA序列测定，定名为枯草芽孢杆菌BS-2018(Bacillus subtilis)。将该菌制成微生物制剂(200×108 cfu/mL)，在海参浮游期(10～15 mL/m3水体/d泼洒)、稚幼参期(饵料中1%～2%添加和5～10 mL/m3水体/d泼洒)、越冬苗保苗期(饵料中1%～2%添加和5～10 m L/m3水体/d泼洒)、成参养殖期(腐植酸钠5～10 g和10～15 m L/m3水体/15 d泼洒)进行应用试验。结果表明：该菌株安全可靠，能使育苗浮游期海参成活率分别提高到22.3%、35.3%，发病率分别降低到18.0%、26.0%；稚幼参期增产15.25%～18.14%；越冬苗保苗期增产11.18%～16.77%；成参养殖期增产11.32%～17.82%。枯草芽孢杆菌BS-2018生物制剂在海参育苗和养殖中应用具有广阔的前景。
　　재동경122°21′，북위39°34′대련황해해역적저니중분리득도일주해양세균，경계통적미생물학감정급16SrDNA서렬측정，정명위고초아포간균BS-2018(Bacillus subtilis)。장해균제성미생물제제(200×108 cfu/mL)，재해삼부유기(10～15 mL/m3수체/d발쇄)、치유삼기(이료중1%～2%첨가화5～10 mL/m3수체/d발쇄)、월동묘보묘기(이료중1%～2%첨가화5～10 m L/m3수체/d발쇄)、성삼양식기(부식산납5～10 g화10～15 m L/m3수체/15 d발쇄)진행응용시험。결과표명：해균주안전가고，능사육묘부유기해삼성활솔분별제고도22.3%、35.3%，발병솔분별강저도18.0%、26.0%；치유삼기증산15.25%～18.14%；월동묘보묘기증산11.18%～16.77%；성삼양식기증산11.32%～17.82%。고초아포간균BS-2018생물제제재해삼육묘화양식중응용구유엄활적전경。
One strain of marine bacterium BS-2018 was isolated from the sediments of the Yellow Sea near Dalian (on-gitude 122 ° 21’ , north latitude 39 ° 34’). It was identiifed as Bacillus subtilis determined by the system microbiology study and (16SrDNA) sequence. Microbial agent (made of BS-2018) and its application test were carried out in the sea cucumber nursery planktonic period (10~15 mL/m3 waters/d spilling), the juvenile period (1%~2%in the bait add and 5~10 mL/m3 waters/d spilling), winter seedling stage (1%~2%in the diets add 5~10 mL/m3 waters/d spilling) and the parameter breeding period (5~10 g humic acid sodium and 10~15 mL/m3 waters/15 d). The results showed that the strain is safe and reliable, the survival rate of nursery planktonic period of sea cucumber increased 22.3%~35.3%, and the incidence rate decreased 18.0%~26.0%;the output of young sea cucumber in juvenile parameters period increased 15.25%~18.14%;winter seedling stage output increased 11.18%~16.77%;farming period yield increased 11.32%~17.82%. It indicated that Bacillus subtilis BS-2018 biological agent in the sea cucumber seedlings and breeding applica-tion has broad prospects.