癌变·畸变·突变
癌變·畸變·突變
암변·기변·돌변
CARCINOGENSES,TERATOGENSIS AND MUTAGENESIS
2014年
2期
144-146
,共3页
李学敏%李建国%李淑琴%边林秀
李學敏%李建國%李淑琴%邊林秀
리학민%리건국%리숙금%변림수
麦绿素%致畸试验%微核试验%小鼠%大鼠
麥綠素%緻畸試驗%微覈試驗%小鼠%大鼠
맥록소%치기시험%미핵시험%소서%대서
barley green%teratogenicity test%micronucleus test%rats%mice
目的：评价麦绿素有无致畸和致突变作用。方法：采用小鼠急性经口毒性试验、小鼠微核试验和大鼠致畸试验检测麦绿素有无致畸和致突变作用。急性经口毒性试验中选用最大耐受剂量法(MTD)，剂量为15 g/kg；微核试验中设麦绿素1.25、2.5和5.0 g/kg剂量组、阴性和阳性对照组，通过两次(间隔24 h)给小鼠灌胃给予受试物后，取其股骨骨髓涂片，染色观察其嗜多染红细胞中微核发生率；致畸试验中设麦绿素1.25、2.5、5.0 g/kg组、阴性和阳性对照组，于Wistar大鼠受孕第7天给受试物，连续10 d，于受孕第20天处死孕鼠，观察胎鼠的外观，生长发育情况，固定后检测胎鼠骨骼和内脏畸形种类，计算畸形率。结果：急性毒性试验中麦绿素对雌雄小鼠经口MTD均大于15 g/kg，属无毒级；小鼠骨髓细胞微核试验中各剂量组微核率与阴性对照组比较，差异均无统计学意义(P>0.05)，而阳性对照组微核率与阴性对照组及各剂量组比较差异均有统计学意义(均<0.01)；大鼠致畸试验中，与阴性对照组比较，各剂量组孕鼠与胎鼠的生长发育差异均无统计学意义(P>0.05)，胎鼠的畸形率亦无统计学意义(P>0.05)，而阳性对照组胎鼠内脏、骨骼畸形率与阴性对照组及各剂量组比较差异均有统计学意义(P均<0.01)。结论：麦绿素对大鼠无致畸作用，对小鼠无致突变作用。P
目的：評價麥綠素有無緻畸和緻突變作用。方法：採用小鼠急性經口毒性試驗、小鼠微覈試驗和大鼠緻畸試驗檢測麥綠素有無緻畸和緻突變作用。急性經口毒性試驗中選用最大耐受劑量法(MTD)，劑量為15 g/kg；微覈試驗中設麥綠素1.25、2.5和5.0 g/kg劑量組、陰性和暘性對照組，通過兩次(間隔24 h)給小鼠灌胃給予受試物後，取其股骨骨髓塗片，染色觀察其嗜多染紅細胞中微覈髮生率；緻畸試驗中設麥綠素1.25、2.5、5.0 g/kg組、陰性和暘性對照組，于Wistar大鼠受孕第7天給受試物，連續10 d，于受孕第20天處死孕鼠，觀察胎鼠的外觀，生長髮育情況，固定後檢測胎鼠骨骼和內髒畸形種類，計算畸形率。結果：急性毒性試驗中麥綠素對雌雄小鼠經口MTD均大于15 g/kg，屬無毒級；小鼠骨髓細胞微覈試驗中各劑量組微覈率與陰性對照組比較，差異均無統計學意義(P>0.05)，而暘性對照組微覈率與陰性對照組及各劑量組比較差異均有統計學意義(均<0.01)；大鼠緻畸試驗中，與陰性對照組比較，各劑量組孕鼠與胎鼠的生長髮育差異均無統計學意義(P>0.05)，胎鼠的畸形率亦無統計學意義(P>0.05)，而暘性對照組胎鼠內髒、骨骼畸形率與陰性對照組及各劑量組比較差異均有統計學意義(P均<0.01)。結論：麥綠素對大鼠無緻畸作用，對小鼠無緻突變作用。P
목적：평개맥록소유무치기화치돌변작용。방법：채용소서급성경구독성시험、소서미핵시험화대서치기시험검측맥록소유무치기화치돌변작용。급성경구독성시험중선용최대내수제량법(MTD)，제량위15 g/kg；미핵시험중설맥록소1.25、2.5화5.0 g/kg제량조、음성화양성대조조，통과량차(간격24 h)급소서관위급여수시물후，취기고골골수도편，염색관찰기기다염홍세포중미핵발생솔；치기시험중설맥록소1.25、2.5、5.0 g/kg조、음성화양성대조조，우Wistar대서수잉제7천급수시물，련속10 d，우수잉제20천처사잉서，관찰태서적외관，생장발육정황，고정후검측태서골격화내장기형충류，계산기형솔。결과：급성독성시험중맥록소대자웅소서경구MTD균대우15 g/kg，속무독급；소서골수세포미핵시험중각제량조미핵솔여음성대조조비교，차이균무통계학의의(P>0.05)，이양성대조조미핵솔여음성대조조급각제량조비교차이균유통계학의의(균<0.01)；대서치기시험중，여음성대조조비교，각제량조잉서여태서적생장발육차이균무통계학의의(P>0.05)，태서적기형솔역무통계학의의(P>0.05)，이양성대조조태서내장、골격기형솔여음성대조조급각제량조비교차이균유통계학의의(P균<0.01)。결론：맥록소대대서무치기작용，대소서무치돌변작용。P
OBJECTIVE: To evaluate the teratogenic and mutagenic effects of barley green by traditional teratogenicity test and micronucleus test. METHODS:Acute toxicity test:the method of maximum tolerated dose (15 g/kg) was used;micronucleus test:five groups (1.25,2.5,5.0 g/kg barley green dose groups,positive and negative groups) were divided. Marrow smear of the femur bone was stained after two treatments 24 h apart,and the rate of micronucleus was calculated. Teratogenicity test:five groups (1.25,2.5,5.0 g/kg barley green dose groups,positive th th and negative groups) were also divided. Rats were treated on the 7 day of pregnancy for10 days. On the 20 day of pregnancy,the appearance,growth and development of fetal rats were checked. In addition,the skeletal and visceral teratogenic changes were also assessed. RESULTS:Acute toxicity test:the level of oral barley green MTD was greater than 15 g/kg. Micronucleus test:bone marrow cell micronucleus rate in each dose group was not different compared with the negative control group (P>0.05),but significantly different compared with the positive control group (P<0.01). Teratogenicity test:the appearance,growth and development,the skeletal and visceral teratogenic changes were not different compared with the negative control group (P>0.05),but significantly different compared with the positive control group (P<0.01). CONCLUSION:The teratogenic and mutagenic effects of barley green were not observed.
