中成药
中成藥
중성약
CHINESE TRADITIONAL PATENT MEDICINE
2009年
12期
1860-1862
,共3页
三黄片%盐酸小檗碱%盐酸巴马汀%土大黄苷%HPLC
三黃片%鹽痠小檗堿%鹽痠巴馬汀%土大黃苷%HPLC
삼황편%염산소벽감%염산파마정%토대황감%HPLC
Sanhuang Tablets%berberine hydrochloride%palmatine hydrochloride%rhaponticin%RP-HPLC
目的:用RP-HPLC法同时测定三黄片(大黄、盐酸小檗碱、黄芩浸膏) 中盐酸小檗碱、盐酸巴马汀、土大黄什的含量.方法:色谱柱:Shimadzu色谱柱(250 mm ×4.6 mm,5 μm);流动相为乙腈-0.05%十二烷基硫酸钠(含0.1%磷酸),梯度洗脱;流速:1.0 mL/min;检测波长:334 nm,柱温:25℃.结果:盐酸小檗碱、盐酸巴马汀、土大黄苷分别在0.05～1.04μg(r=0.999 9),0.02～0.49μg(r=O.999 8),0.05～1.02μg(r=0.999 9),范围内线性关系良好;3个成分的平均回收率(n=6)分别为98.7%,97.2%,99.3%.结论:本方法准确可靠,适用于三黄片的质量控制.
目的:用RP-HPLC法同時測定三黃片(大黃、鹽痠小檗堿、黃芩浸膏) 中鹽痠小檗堿、鹽痠巴馬汀、土大黃什的含量.方法:色譜柱:Shimadzu色譜柱(250 mm ×4.6 mm,5 μm);流動相為乙腈-0.05%十二烷基硫痠鈉(含0.1%燐痠),梯度洗脫;流速:1.0 mL/min;檢測波長:334 nm,柱溫:25℃.結果:鹽痠小檗堿、鹽痠巴馬汀、土大黃苷分彆在0.05～1.04μg(r=0.999 9),0.02～0.49μg(r=O.999 8),0.05～1.02μg(r=0.999 9),範圍內線性關繫良好;3箇成分的平均迴收率(n=6)分彆為98.7%,97.2%,99.3%.結論:本方法準確可靠,適用于三黃片的質量控製.
목적:용RP-HPLC법동시측정삼황편(대황、염산소벽감、황금침고) 중염산소벽감、염산파마정、토대황십적함량.방법:색보주:Shimadzu색보주(250 mm ×4.6 mm,5 μm);류동상위을정-0.05%십이완기류산납(함0.1%린산),제도세탈;류속:1.0 mL/min;검측파장:334 nm,주온:25℃.결과:염산소벽감、염산파마정、토대황감분별재0.05～1.04μg(r=0.999 9),0.02～0.49μg(r=O.999 8),0.05～1.02μg(r=0.999 9),범위내선성관계량호;3개성분적평균회수솔(n=6)분별위98.7%,97.2%,99.3%.결론:본방법준학가고,괄용우삼황편적질량공제.
