CAJ | 학술논문

目前生物柴油的价格是石化柴油的1.5倍，寻求新的廉价易得的原料降低生物柴油的成本是必然趋势。作物秸秆是一种低廉普遍的废弃物，秸秆中含有纤维素，半纤维素等成分，水解成单糖后可以作为合成生物柴油所需的糖类来源。利用前期研究构建的基因工程菌Escherichia coli pET28a（＋）-PAW发酵秸秆（玉米秸秆和小麦秸秆）水解液得到乙醇，并在胞内将乙醇与外源添加的脂肪酸进行同步转化合成生物柴油的主要成分脂肪酸乙酯。 HPLC分析表明玉米水解液中葡萄糖含量（10.40 g?L-1）较高，小麦水解液中木糖含量（40.56 g?L-1）较高。 E．coli pET28a（＋）-PAW能够有效地以小麦和玉米两种秸秆水解液作为糖类替代物发酵生产生物柴油，小麦水解液培养基中的生物柴油含量（0.30 g?L-1）高于玉米水解液培养基中的含量（0.25 g?L-1），同时也高于现有报道。结果表明，以秸秆水解液作为原料，利用基因工程菌合成生物柴油是可行的，有助于降低生物柴油的原料成本。
목전생물시유적개격시석화시유적1.5배，심구신적렴개역득적원료강저생물시유적성본시필연추세。작물갈간시일충저렴보편적폐기물，갈간중함유섬유소，반섬유소등성분，수해성단당후가이작위합성생물시유소수적당류래원。이용전기연구구건적기인공정균Escherichia coli pET28a（＋）-PAW발효갈간（옥미갈간화소맥갈간）수해액득도을순，병재포내장을순여외원첨가적지방산진행동보전화합성생물시유적주요성분지방산을지。 HPLC분석표명옥미수해액중포도당함량（10.40 g?L-1）교고，소맥수해액중목당함량（40.56 g?L-1）교고。 E．coli pET28a（＋）-PAW능구유효지이소맥화옥미량충갈간수해액작위당류체대물발효생산생물시유，소맥수해액배양기중적생물시유함량（0.30 g?L-1）고우옥미수해액배양기중적함량（0.25 g?L-1），동시야고우현유보도。결과표명，이갈간수해액작위원료，이용기인공정균합성생물시유시가행적，유조우강저생물시유적원료성본。
The price of biodiesel is approximately 1.5 times higher than that of traditional diesel .It is urgent to explore cheaply available substrates to reduce the cost of biodiesel production .As one of the cheap and widespread wastes, lignocellulose contained cellulose and hemicellulose which could be hydrolyzed and used in the fermentation of biodiesel.The genetic engineered strain Escherichia coli pET28a (+)-PAW fermented straw hydrolysates into alcohol, and further into biodiesel with fatty acids by one-step transformation in-vivo.The HPLC analysis showed that the corn straw hydrolysate had a higher glucose concentration (10.40 g?L-1 ) and the wheat straw hydrolysate had a higher xylose concentration (40.56 g?L-1 ).When straw hydrolysates were utilized as sugar substitutes for biodiesel production by E.coli pET28a(+)-PAW, the maximum biodiesel yield of 0.30 g? L-1 ( wheat hydrolysate ) was obtained which is higher than the yield of biodiesel 0.25 g?L-1 (corn hydrolysate) and the present reports.The straw hydrolysates could be utilized by genetic engineering strain as the substrates for biodiesel production , to reduce the material price of biodiesel.