中华老年多器官疾病杂志
中華老年多器官疾病雜誌
중화노년다기관질병잡지
CHINESE JOURNAL OF MULTIPLE ORGAN DISEASES IN THE ELDERLY
2013年
7期
534-537
,共4页
认知功能障碍%七氟烷%Morris水迷宫%磷酸丙酮酸水合酶
認知功能障礙%七氟烷%Morris水迷宮%燐痠丙酮痠水閤酶
인지공능장애%칠불완%Morris수미궁%린산병동산수합매
cognitive dysfunction%sevoflurane%Morris water maze%neuron specific enolase
目的探讨不同浓度（1%，2%，3%）七氟烷对成年大鼠的认知功能及血清、海马组织中神经元特异性烯醇化酶（NSE）的影响。方法将40只成年雄性SD大鼠随机分为1%七氟烷组、2%七氟烷组、3%七氟烷组及对照组（吸入空气），七氟烷吸入3h。处理后第1，2，3，4，5天进行Morris水迷宫的定位航行试验和第6天的空间探索试验。第6天测试完毕后取大鼠的血清和海马组织测定NSE的含量。结果吸入不同浓度七氟烷组与对照组相同时间点比较，逃避潜伏期和总路程差异无统计学意义（P＞0.05）。同时，不同浓度七氟烷组间比较，逃避潜伏期和总路程差异也无统计学意义（P＞0.05）。吸入七氟烷或空气后第6天撤台，各组大鼠的目标象限停留时间百分比和穿越平台次数之间差异也无统计学意义（P＞0.05）。吸入1%和3%七氟烷组大鼠血清中NSE水平低于对照组（P＜0.01）；同时，吸入2%和3%七氟烷组大鼠血清中NSE水平高于吸入1%七氟烷组（P＜0.01，P＜0.05）。海马组织中的NSE含量各组间差异无统计学意义（P＞0.05）。结论单次吸入1%，2%和3%的七氟烷，短期不影响成年大鼠的空间学习记忆能力。同时，吸入1%和3%七氟烷可以降低大鼠血清中NSE水平。
目的探討不同濃度（1%，2%，3%）七氟烷對成年大鼠的認知功能及血清、海馬組織中神經元特異性烯醇化酶（NSE）的影響。方法將40隻成年雄性SD大鼠隨機分為1%七氟烷組、2%七氟烷組、3%七氟烷組及對照組（吸入空氣），七氟烷吸入3h。處理後第1，2，3，4，5天進行Morris水迷宮的定位航行試驗和第6天的空間探索試驗。第6天測試完畢後取大鼠的血清和海馬組織測定NSE的含量。結果吸入不同濃度七氟烷組與對照組相同時間點比較，逃避潛伏期和總路程差異無統計學意義（P＞0.05）。同時，不同濃度七氟烷組間比較，逃避潛伏期和總路程差異也無統計學意義（P＞0.05）。吸入七氟烷或空氣後第6天撤檯，各組大鼠的目標象限停留時間百分比和穿越平檯次數之間差異也無統計學意義（P＞0.05）。吸入1%和3%七氟烷組大鼠血清中NSE水平低于對照組（P＜0.01）；同時，吸入2%和3%七氟烷組大鼠血清中NSE水平高于吸入1%七氟烷組（P＜0.01，P＜0.05）。海馬組織中的NSE含量各組間差異無統計學意義（P＞0.05）。結論單次吸入1%，2%和3%的七氟烷，短期不影響成年大鼠的空間學習記憶能力。同時，吸入1%和3%七氟烷可以降低大鼠血清中NSE水平。
목적탐토불동농도（1%，2%，3%）칠불완대성년대서적인지공능급혈청、해마조직중신경원특이성희순화매（NSE）적영향。방법장40지성년웅성SD대서수궤분위1%칠불완조、2%칠불완조、3%칠불완조급대조조（흡입공기），칠불완흡입3h。처리후제1，2，3，4，5천진행Morris수미궁적정위항행시험화제6천적공간탐색시험。제6천측시완필후취대서적혈청화해마조직측정NSE적함량。결과흡입불동농도칠불완조여대조조상동시간점비교，도피잠복기화총로정차이무통계학의의（P＞0.05）。동시，불동농도칠불완조간비교，도피잠복기화총로정차이야무통계학의의（P＞0.05）。흡입칠불완혹공기후제6천철태，각조대서적목표상한정류시간백분비화천월평태차수지간차이야무통계학의의（P＞0.05）。흡입1%화3%칠불완조대서혈청중NSE수평저우대조조（P＜0.01）；동시，흡입2%화3%칠불완조대서혈청중NSE수평고우흡입1%칠불완조（P＜0.01，P＜0.05）。해마조직중적NSE함량각조간차이무통계학의의（P＞0.05）。결론단차흡입1%，2%화3%적칠불완，단기불영향성년대서적공간학습기억능력。동시，흡입1%화3%칠불완가이강저대서혈청중NSE수평。
Objective To determine the effect of sevoflurane at different doses (1%, 2%, 3%) on cognitive function and contents of neuron specific enolase (NSE) in the serum and hippocampus in adult rats. Methods Forty male adult Sprague Dawley(SD) rats were randomly divided into the following three treatment groups and control. The rats from the three treatment groups were exposed to 1%, 2%and 3%sevoflurane for 3h, respectively, while those of control were exposed to air. The navigation tests in Morris water maze (MWM) were performed on day 1, 2, 3, 4 and 5 after the treatment, and the pilot tests in MWM were performed on day 6. Blood samples and hippocampal tissues were collected from the rats in each group after the pilot tests on day 6 after the measurement for the content of NSE by enzyme linked immunosorbent assay. Results No significant difference was found in the escape latency and path length between the sevoflurane groups and control (P>0.05), and among sevoflurane groups of different doses (P>0.05). There was no significant difference in the mean percentage of crossing platform quadrant and the frequency of crossing platform among the four groups (P>0.05). The serum level of NSE was significantly lower in the 1%and 3%sevoflurane groups than in the control (P<0.01), and it was obviously higher in the 2% and 3% sevoflurane groups than in the 1% sevoflurane group (P<0.05 or P<0.01). No significant difference was seen in the hippocampal content of NSE in the rats from the four groups (P>0.05). Conclusion The single exposure of sevoflurane at different doses has no short-term impact on the learning and memory in adult rats. The serum NSE level is decreased after exposure to 1%and 3%sevoflurane.
