中华老年多器官疾病杂志
中華老年多器官疾病雜誌
중화노년다기관질병잡지
CHINESE JOURNAL OF MULTIPLE ORGAN DISEASES IN THE ELDERLY
2013年
7期
530-533
,共4页
睾酮%高血压%血管内皮生长因子类%实时聚合酶链反应
睪酮%高血壓%血管內皮生長因子類%實時聚閤酶鏈反應
고동%고혈압%혈관내피생장인자류%실시취합매련반응
testosterone%hypertension%vascular endothelial growth factors%real-time polymerase chain reaction
目的探讨不同水平睾酮对高盐饮食下雄性大鼠血管内皮生长因子的影响。方法雄性Wistar大鼠32只随机分为4组：假手术组（Sham组），去势组（Cas组），去势后补充睾酮组（TC组），去势后补充睾酮和氟他胺组（FTC组）。每组8只。各组大鼠均以8%氯化钠高盐颗粒饲料喂养；Sham组施以假手术，Cas、TC及FTC组均施以去势手术。TC组及FTC组予丙酸睾酮2mg/kg、隔日1次皮下注射，FTC组同时予氟他胺1mg/kg、每日1次灌胃，持续8周。8周后采用酶联免疫吸附测定法（ELISA）测定各组血清中睾酮、血管内皮生长因子（VEGF）的浓度，实时定量PCR（RT-PCR）测定血管内皮中VEGF mRNA的表达水平。结果（1）Cas组睾酮浓度[（0.26±0.02）μg/L]与Sham组[（3.15±0.54）μg/L]相比明显下降，而FTC组[（5.47±1.29）μg/L]、TC组[（2.80±0.30）ng/L]则明显升高（均P＜0.01）；（2）与Sham组的血清VEGF浓度[（20.837±13.823）ng/L]相比，Cas组[（47.246±19.733）ng/L， P＜0.01]和FTC组[（46.043±22.607）ng/L，P＜0.05]明显升高，TC组[（22.870±12.057）ng/L]则无明显差异（P＞0.05）；（3）与Sham组相比，Cas组和FTC组血管内皮细胞VEGF mRNA表达水平均显著降低（均P＜0.01），而TC组无显著差异（P＞0.05）。结论高盐饮食下，睾酮水平增高抑制血管内皮生长因子的表达和释放。
目的探討不同水平睪酮對高鹽飲食下雄性大鼠血管內皮生長因子的影響。方法雄性Wistar大鼠32隻隨機分為4組：假手術組（Sham組），去勢組（Cas組），去勢後補充睪酮組（TC組），去勢後補充睪酮和氟他胺組（FTC組）。每組8隻。各組大鼠均以8%氯化鈉高鹽顆粒飼料餵養；Sham組施以假手術，Cas、TC及FTC組均施以去勢手術。TC組及FTC組予丙痠睪酮2mg/kg、隔日1次皮下註射，FTC組同時予氟他胺1mg/kg、每日1次灌胃，持續8週。8週後採用酶聯免疫吸附測定法（ELISA）測定各組血清中睪酮、血管內皮生長因子（VEGF）的濃度，實時定量PCR（RT-PCR）測定血管內皮中VEGF mRNA的錶達水平。結果（1）Cas組睪酮濃度[（0.26±0.02）μg/L]與Sham組[（3.15±0.54）μg/L]相比明顯下降，而FTC組[（5.47±1.29）μg/L]、TC組[（2.80±0.30）ng/L]則明顯升高（均P＜0.01）；（2）與Sham組的血清VEGF濃度[（20.837±13.823）ng/L]相比，Cas組[（47.246±19.733）ng/L， P＜0.01]和FTC組[（46.043±22.607）ng/L，P＜0.05]明顯升高，TC組[（22.870±12.057）ng/L]則無明顯差異（P＞0.05）；（3）與Sham組相比，Cas組和FTC組血管內皮細胞VEGF mRNA錶達水平均顯著降低（均P＜0.01），而TC組無顯著差異（P＞0.05）。結論高鹽飲食下，睪酮水平增高抑製血管內皮生長因子的錶達和釋放。
목적탐토불동수평고동대고염음식하웅성대서혈관내피생장인자적영향。방법웅성Wistar대서32지수궤분위4조：가수술조（Sham조），거세조（Cas조），거세후보충고동조（TC조），거세후보충고동화불타알조（FTC조）。매조8지。각조대서균이8%록화납고염과립사료위양；Sham조시이가수술，Cas、TC급FTC조균시이거세수술。TC조급FTC조여병산고동2mg/kg、격일1차피하주사，FTC조동시여불타알1mg/kg、매일1차관위，지속8주。8주후채용매련면역흡부측정법（ELISA）측정각조혈청중고동、혈관내피생장인자（VEGF）적농도，실시정량PCR（RT-PCR）측정혈관내피중VEGF mRNA적표체수평。결과（1）Cas조고동농도[（0.26±0.02）μg/L]여Sham조[（3.15±0.54）μg/L]상비명현하강，이FTC조[（5.47±1.29）μg/L]、TC조[（2.80±0.30）ng/L]칙명현승고（균P＜0.01）；（2）여Sham조적혈청VEGF농도[（20.837±13.823）ng/L]상비，Cas조[（47.246±19.733）ng/L， P＜0.01]화FTC조[（46.043±22.607）ng/L，P＜0.05]명현승고，TC조[（22.870±12.057）ng/L]칙무명현차이（P＞0.05）；（3）여Sham조상비，Cas조화FTC조혈관내피세포VEGF mRNA표체수평균현저강저（균P＜0.01），이TC조무현저차이（P＞0.05）。결론고염음식하，고동수평증고억제혈관내피생장인자적표체화석방。
Objective To determine the effect of different doses of testosterone on vascular endothelial growth factor (VEGF) in high-salt fed rats. Methods Thirty-two Wistar male rats were randomly assigned to 4 groups, sham-operation (Sham) group, castration (Cas) group, testosterone supplement after castration (TC) group, and flutamide-treated testosterone supplemented castrated (FTC) group, with 8 rats in each group. After all animals were fed with high-salt diet (8% NaCl) for 8 weeks, the rats of Sham group received a sham operation, while those in Cas, TC and FTC groups received surgical castration. Testosterone propionate (TP) was injected subcutaneously at 2mg/kg, once every 2 days in TC and FTC groups, and flutamide 1mg/(kg·d) was given intragastrically in FTC group, for 8 weeks after surgery. Serum levels of testosterone and VEGF were determined by enzyme-linked immunosorbent assay (ELISA). Expression of VEGF mRNA in the blood samples were determined by real-time PCR (RT-PCR). Results Serum levels of testosterone was obviously lower in Cas group, but significantly higher in TC and FTC groups when compared with Sham group [(0.26±0.02), (2.80±0.30), (5.47±1.29) vs (3.15±0.54)μg/ml, P<0.01]. For serum level of VEGF, Cas FTC groups had obviously increased levels than Sham group [(47.246±19.733), (46.043±22.607) vs (20.837±13.823) ng/L, P<0.01, P<0.05], but no difference was found between Sham group and TC group [ (22.870±12.057)ng/L, P>0.05]. Cas group and FTC group had lower expression of VEGF mRNA compared with Sham group (P<0.01), but no difference with TC group(P>0.05). Conclusion Under high-salt diet, high level of testosterone suppresses the expression and release of VEGF.
