南昌大学学报(医学版)
南昌大學學報(醫學版)
남창대학학보(의학판)
ACTA ACADEMIAE MEDICINAE JIANGXI
2014年
2期
31-34
,共4页
血培养%病原菌%耐药性
血培養%病原菌%耐藥性
혈배양%병원균%내약성
blood culture%pathogens%drug resistance
目的:了解内蒙古自治区人民医院2009-2012年血培养阳性标本的病原菌分布和耐药性变迁。方法采用 Bact/Alert 3D 全自动血培养仪,对血培养培养瓶进行连续培养监测,细菌鉴定和药敏试验采用法国生物梅里埃公司 VITEK 2-compact 全自动微生物分析系统,应用 Whonet5.6软件对数据进行统计分析。结果2009-2012年,该院共分离出659株病原菌,阳性率为13.9%。其中,革兰阴性杆菌和革兰阳性球菌所占比例分别为54.5%、42.9%。大肠埃希菌检出率最高,占25.6%,其次是凝固酶阴性葡萄球菌(人葡萄球菌和表皮葡萄球菌),占23.9%。血培养阳性菌多见于泌尿内科(占12.3%)、ICU(占12.5%)、普外科(占11.2%)。耐药性分析显示:血行感染肠杆菌科细菌对亚胺培南高度敏感(100.0%),其对头孢替坦、哌拉西林/他唑巴坦和阿米卡星也较敏感(>85.7%)。未发现耐万古霉素的葡萄球菌和肠球菌,利奈唑胺和奎奴普丁/达福普丁也对革兰阳性球菌有较好的敏感性(>93.1%)。铜绿假单胞菌和不动杆菌对多种抗菌药物的耐药率比较高,只有阿米卡星、哌拉西林/他唑巴坦、氨苄西林/舒巴坦、环丙沙星和左旋氧氟沙星耐药率较低(<17.1%)。结论血培养病原菌分布和耐药性变迁有其特点,可为其治疗及预防提供依据。
目的:瞭解內矇古自治區人民醫院2009-2012年血培養暘性標本的病原菌分佈和耐藥性變遷。方法採用 Bact/Alert 3D 全自動血培養儀,對血培養培養瓶進行連續培養鑑測,細菌鑒定和藥敏試驗採用法國生物梅裏埃公司 VITEK 2-compact 全自動微生物分析繫統,應用 Whonet5.6軟件對數據進行統計分析。結果2009-2012年,該院共分離齣659株病原菌,暘性率為13.9%。其中,革蘭陰性桿菌和革蘭暘性毬菌所佔比例分彆為54.5%、42.9%。大腸埃希菌檢齣率最高,佔25.6%,其次是凝固酶陰性葡萄毬菌(人葡萄毬菌和錶皮葡萄毬菌),佔23.9%。血培養暘性菌多見于泌尿內科(佔12.3%)、ICU(佔12.5%)、普外科(佔11.2%)。耐藥性分析顯示:血行感染腸桿菌科細菌對亞胺培南高度敏感(100.0%),其對頭孢替坦、哌拉西林/他唑巴坦和阿米卡星也較敏感(>85.7%)。未髮現耐萬古黴素的葡萄毬菌和腸毬菌,利奈唑胺和奎奴普丁/達福普丁也對革蘭暘性毬菌有較好的敏感性(>93.1%)。銅綠假單胞菌和不動桿菌對多種抗菌藥物的耐藥率比較高,隻有阿米卡星、哌拉西林/他唑巴坦、氨芐西林/舒巴坦、環丙沙星和左鏇氧氟沙星耐藥率較低(<17.1%)。結論血培養病原菌分佈和耐藥性變遷有其特點,可為其治療及預防提供依據。
목적:료해내몽고자치구인민의원2009-2012년혈배양양성표본적병원균분포화내약성변천。방법채용 Bact/Alert 3D 전자동혈배양의,대혈배양배양병진행련속배양감측,세균감정화약민시험채용법국생물매리애공사 VITEK 2-compact 전자동미생물분석계통,응용 Whonet5.6연건대수거진행통계분석。결과2009-2012년,해원공분리출659주병원균,양성솔위13.9%。기중,혁란음성간균화혁란양성구균소점비례분별위54.5%、42.9%。대장애희균검출솔최고,점25.6%,기차시응고매음성포도구균(인포도구균화표피포도구균),점23.9%。혈배양양성균다견우비뇨내과(점12.3%)、ICU(점12.5%)、보외과(점11.2%)。내약성분석현시:혈행감염장간균과세균대아알배남고도민감(100.0%),기대두포체탄、고랍서림/타서파탄화아미잡성야교민감(>85.7%)。미발현내만고매소적포도구균화장구균,리내서알화규노보정/체복보정야대혁란양성구균유교호적민감성(>93.1%)。동록가단포균화불동간균대다충항균약물적내약솔비교고,지유아미잡성、고랍서림/타서파탄、안변서림/서파탄、배병사성화좌선양불사성내약솔교저(<17.1%)。결론혈배양병원균분포화내약성변천유기특점,가위기치료급예방제공의거。
Objective To investigate the distribution and drug resistance changes of blood cul-ture pathogens in Inner Mongolian People’s Hospital between 2009 and 2012.Methods Blood samples were cultured with the BacT/Alert 3D automated blood culture system.Bacterial identifi-cation and antibiotic susceptibility testing were performed using Vitek 2 system(bio-Mérieux, France).All data were analyzed using Whonet 5.6 software.Results A total of 659 strains of pathogens were isolated from blood samples between 2009 and 2012.The positive rate was 13.9%.Of the 659 strains,gram-negative bacilli and gram-positive cocci accounted for 54.5% and 42.9%,respectively.The detection rate was highest in Escherichia coli(25.6%),followed by co-agulase-negative staphylococcus(Staphylococcus aureus and staphylococcus epidermidis,23.9%). The positive blood culture pathogens were commonly found in department of urology(12.3%), ICU(12.5%) and department of general surgery (11.2%).Antibiotic susceptibility testing showed that Enterobacteriaceae strains were highly susceptible to carbapenem (100.0%),and were also sensitive to cefotetan,piperacillin/tazobactam and amikacin(>85.7%).No strains re-sistant to vancomycin were found in Staphylococcus and Enterococcus isolates.Linezolid and quinu-pristin/dalfopristin exhibited high activity against gram-positive cocci(>93.1%).Pseudomonas aerugi-nosa and Acinetobacter were highly resistant to multiple antimicrobial agents,except for amikacin,pip-eracillin/tazobactam,ampicillin/sulbactam,ciprofloxacin and levofloxacin(<17.1%).Conclusion The distribution and drug resistance changes of blood culture pathogens have their own characterris-tics,which can provide the basis for the treatment and prevention of diseases.