茶叶通讯
茶葉通訊
다협통신
TEA COMMUNICATION
2014年
1期
15-17,23
,共4页
茶黄素%高糖%人晶状体上皮细胞%氧化损伤
茶黃素%高糖%人晶狀體上皮細胞%氧化損傷
다황소%고당%인정상체상피세포%양화손상
Theaflavins%High glucose%Human lens epithelial cells%Oxidative damage
研究茶黄素（Theaflavin，TF）对高糖(High glucose，HG)诱导的人晶状体上皮细胞（Human lens epithelial cells，HLECs）氧化损伤的影响。采用体外培养人晶状体上皮细胞，用葡萄糖诱导细胞成氧化损伤模型，外加一定浓度的茶黄素干预，比色法检测细胞体内SOD、CAT、GSH-Px、MDA活力和含量的变化。结果表明相对于正常培养的细胞，高糖诱导细胞体内SOD、CAT、GSH的活性降低，MDA含量上升；加入茶黄素后，提高了细胞内SOD、CAT、GSH-Px的活力，降低了细胞内MDA的含量，与模型组细胞相比，具有显著差异（P＜0.01）。
研究茶黃素（Theaflavin，TF）對高糖(High glucose，HG)誘導的人晶狀體上皮細胞（Human lens epithelial cells，HLECs）氧化損傷的影響。採用體外培養人晶狀體上皮細胞，用葡萄糖誘導細胞成氧化損傷模型，外加一定濃度的茶黃素榦預，比色法檢測細胞體內SOD、CAT、GSH-Px、MDA活力和含量的變化。結果錶明相對于正常培養的細胞，高糖誘導細胞體內SOD、CAT、GSH的活性降低，MDA含量上升；加入茶黃素後，提高瞭細胞內SOD、CAT、GSH-Px的活力，降低瞭細胞內MDA的含量，與模型組細胞相比，具有顯著差異（P＜0.01）。
연구다황소（Theaflavin，TF）대고당(High glucose，HG)유도적인정상체상피세포（Human lens epithelial cells，HLECs）양화손상적영향。채용체외배양인정상체상피세포，용포도당유도세포성양화손상모형，외가일정농도적다황소간예，비색법검측세포체내SOD、CAT、GSH-Px、MDA활력화함량적변화。결과표명상대우정상배양적세포，고당유도세포체내SOD、CAT、GSH적활성강저，MDA함량상승；가입다황소후，제고료세포내SOD、CAT、GSH-Px적활력，강저료세포내MDA적함량，여모형조세포상비，구유현저차이（P＜0.01）。
To study the effect of theaflavins on oxidative damage in human lens epithelial cells under high glucose. Methods:Human lens epithelial cells were cultured with HG (30mM)to Establish the oxidative damage model. After the culture under suitable temperature for 24h ,32μM and 64μM TF were added into TF-growps. The SOD、CAT、GSH-Px、MDA were measured with using colorimetry. Results:Compared with the normal cultured cells, The SOD, CAT, GSH-Px activity were redused in cells induced by high glucose, the MDA content increased;After add the TF, it improved the SOD, CAT, GSH activity, MDA content decreased(P<0.01). Conclusion: Theaflavins can effectively control the activities of antioxidant enzymes in human lens epithelial cells induced by high glucose .
