CAJ | 학술논문

为在分子水平上鉴定“湘波绿2号”的父本，本研究采用SSR标记对其母本和5个可能父本进行了分析。结果表明，筛选的29对引物共检测到83个等位位点，每个引物1～4个，平均2.86个。湘波绿2号与福鼎大白茶及5个可能父本的相同位点数分别占其扩增总位点数的71.93%、64.91%、94.74%、66.67%、52.63%和70.18%，最多的为湘波绿，最少的为云大72-04。7份供试材料间相似系数变幅为0.45～0.80，平均0.55，其中湘波绿2号与湘波绿的相似系数最大（0.80）。根据相似系数UPGMA聚类，在相似系数0.55处将参试7份材料分为5类。参试材料的相似系数和亲缘关系树状图在分子水平上显示了湘波绿与湘波绿2号的亲缘关系最近，可能为湘波绿2号的父本。
위재분자수평상감정“상파록2호”적부본，본연구채용SSR표기대기모본화5개가능부본진행료분석。결과표명，사선적29대인물공검측도83개등위위점，매개인물1～4개，평균2.86개。상파록2호여복정대백다급5개가능부본적상동위점수분별점기확증총위점수적71.93%、64.91%、94.74%、66.67%、52.63%화70.18%，최다적위상파록，최소적위운대72-04。7빈공시재료간상사계수변폭위0.45～0.80，평균0.55，기중상파록2호여상파록적상사계수최대（0.80）。근거상사계수UPGMA취류，재상사계수0.55처장삼시7빈재료분위5류。삼시재료적상사계수화친연관계수상도재분자수평상현시료상파록여상파록2호적친연관계최근，가능위상파록2호적부본。
SSR primers were used to identify the male parents for Xiangbolv 2 among 5 possible male parents. Totally 83 alleles were amplified using 29 core SSR primers, the number of alleles per primer ranged from 1 to 4, on average of 2.86. The percentage of common alleles with Xiangbolv 2 is 71.93%,64.91%,94.74%,66.67%,52.63%,70.18% each other, the maximum is Xiangbolv, the minimum is Yunda 72-04.The similarity coefficient among different cultivars varied from 0.45 to 0.80, on average of 0.55. The 7 accessions were classified into 5 groups based on the UPGMA method with the similarity coefficient at 0.55. The results of the similarity coefficient and dendrogram showed a closer relationship between Xiangbolv and Xiangbolv 2, Xiangbolv is the possible male parent of Xiangbolv 2.