华南师范大学学报（自然科学版）
華南師範大學學報（自然科學版）
화남사범대학학보（자연과학판）
JOURNAL OF SOUTH CHINA NORMAL UNIVERSITY （NATURAL SCIENCE EDITION）
2014年
2期
99-107
,共9页
王宇涛%陈志勇%曾琬淋%李韶山
王宇濤%陳誌勇%曾琬淋%李韶山
왕우도%진지용%증완림%리소산
拟南芥%镉( Cd)%生理响应
擬南芥%鎘( Cd)%生理響應
의남개%력( Cd)%생리향응
Arabidopsis thaliana%cadmium ( Cd)%physiological response
研究拟南芥对不同Cd2＋浓度处理（0、20、40、60、80μmol/L）的生理响应，探讨Cd2＋对植物的毒性效应及植物的耐性机理．结果发现，各浓度Cd2＋导致拟南芥过氧化氢酶和超氧化物歧化酶活性显著降低（P＜0.05）、植物根部细胞DNA出现明显的损伤，拟南芥生物量及根长也显著降低（P＜0.05），表明Cd2＋对拟南芥体内的生物大分子产生了严重的胁迫效应．叶片光合色素含量随Cd2＋浓度的升高而逐渐下降，在60、80μmol/L时处理效应达到显著水平（P＜0.05），表明高Cd2＋抑制植物的光合作用．拟南芥体内丙二醛及可溶性蛋白含量随Cd2＋浓度的增加明显上升，在60μmol/L时处理效应显著（P＜0.05），表明Cd2＋胁迫导致其膜脂过氧化效应．在全部Cd2＋处理下，拟南芥体内谷胱甘肽和植物螯合肽的含量均显著上升（ P＜0.01）；类黄酮、花色素苷等酚类物质也随Cd2＋浓度的增加而上升，并在高Cd2＋浓度（60、80μmol/L）下处理效应达到显著水平（P＜0.05），表明拟南芥通过积累具有活性氧抗性的次级代谢产物提高其对Cd2＋的耐受性．本研究结果为深入研究Cd2＋对植物毒害的分子机制以及植物对Cd2＋耐性的分子机制提供依据．
研究擬南芥對不同Cd2＋濃度處理（0、20、40、60、80μmol/L）的生理響應，探討Cd2＋對植物的毒性效應及植物的耐性機理．結果髮現，各濃度Cd2＋導緻擬南芥過氧化氫酶和超氧化物歧化酶活性顯著降低（P＜0.05）、植物根部細胞DNA齣現明顯的損傷，擬南芥生物量及根長也顯著降低（P＜0.05），錶明Cd2＋對擬南芥體內的生物大分子產生瞭嚴重的脅迫效應．葉片光閤色素含量隨Cd2＋濃度的升高而逐漸下降，在60、80μmol/L時處理效應達到顯著水平（P＜0.05），錶明高Cd2＋抑製植物的光閤作用．擬南芥體內丙二醛及可溶性蛋白含量隨Cd2＋濃度的增加明顯上升，在60μmol/L時處理效應顯著（P＜0.05），錶明Cd2＋脅迫導緻其膜脂過氧化效應．在全部Cd2＋處理下，擬南芥體內穀胱甘肽和植物螯閤肽的含量均顯著上升（ P＜0.01）；類黃酮、花色素苷等酚類物質也隨Cd2＋濃度的增加而上升，併在高Cd2＋濃度（60、80μmol/L）下處理效應達到顯著水平（P＜0.05），錶明擬南芥通過積纍具有活性氧抗性的次級代謝產物提高其對Cd2＋的耐受性．本研究結果為深入研究Cd2＋對植物毒害的分子機製以及植物對Cd2＋耐性的分子機製提供依據．
연구의남개대불동Cd2＋농도처리（0、20、40、60、80μmol/L）적생리향응，탐토Cd2＋대식물적독성효응급식물적내성궤리．결과발현，각농도Cd2＋도치의남개과양화경매화초양화물기화매활성현저강저（P＜0.05）、식물근부세포DNA출현명현적손상，의남개생물량급근장야현저강저（P＜0.05），표명Cd2＋대의남개체내적생물대분자산생료엄중적협박효응．협편광합색소함량수Cd2＋농도적승고이축점하강，재60、80μmol/L시처리효응체도현저수평（P＜0.05），표명고Cd2＋억제식물적광합작용．의남개체내병이철급가용성단백함량수Cd2＋농도적증가명현상승，재60μmol/L시처리효응현저（P＜0.05），표명Cd2＋협박도치기막지과양화효응．재전부Cd2＋처리하，의남개체내곡광감태화식물오합태적함량균현저상승（ P＜0.01）；류황동、화색소감등분류물질야수Cd2＋농도적증가이상승，병재고Cd2＋농도（60、80μmol/L）하처리효응체도현저수평（P＜0.05），표명의남개통과적루구유활성양항성적차급대사산물제고기대Cd2＋적내수성．본연구결과위심입연구Cd2＋대식물독해적분자궤제이급식물대Cd2＋내성적분자궤제제공의거．
To illustrate the toxic effects of Cd 2+on plants and their tolerance mechanisms , the physiological respon-ses of Arabidopsis thaliana L.under different Cd2+treatments (0, 20, 40, 60 and 80μmol/L) were investigated. The results showed that compared to control plants , there were significantly lower biomass and root length of plants in Cd2+treatments (P<0.05).The application of Cd2+induced obvious DNA injury in plant roots, and caused significant inhibitory effects on SOD and POD activities ( P<0.05 ) .20μmol/L or higher Cd 2+concentrations can lead to high oxidative stresses on the biomacromolecules of plant .The photosynthetic pigments contents of A.thali-ana were gradually decreased following increased Cd 2+, and statistically significant effects were observed under 60 and 80 μmol/L Cd2+treatments (P<0.05), indicating that the high Cd2+treatments inhibited photosynthesis of plants.The soluble protein and MDA contents increased obviously following increased Cd 2 +concentrations, and significantly higher contents of them were observed in 60 and 80 μmol/L Cd2+treatments than that in the control ( P<0.05 ) , indicating that membrane lipid peroxidation were induced by high Cd 2+application .The glutathione and phytochelatin contents of plant showed significant increases following increased Cd 2 + concentrations ( P <0.05 ) .There were also higher contents of flavonoid and anthocyanin in the plants under Cd 2+treatments , indica-ting that accumulation of secondary metabolites with antioxidant activities was one of the strategies in A.thaliana to improve their Cd2+tolerance.The present study could provide experimental data on the exploration of the molecular mechanisms of toxic effects of Cd 2+on plants and their tolerance strategies .
