CAJ | 학술논문

了解我国睑虎属Goniurosaurus （ Squamata：Sauria：Eublepharidae ）种间的亲缘关系以及广东新纪录的睑虎属未定种Goniurosaurus indet．的有效性，对睑虎属10个种的线粒体DNA基因片段比较和遗传变异分析．获得12S rRNA （385 bp）和16S rRNA （477 bp）总长度为862 bp的联合序列，共有核苷酸变异位点388个，简约信息位点269个．选取大壁虎Gekko gecko为外类群，采用贝叶斯演绎法（BI）、最大似然法（ML）、最大简约法（MP）、邻接法（NJ）对两个基因联合序列的分析均显示相同结果，即广东睑虎属未定种Goniurosaurus indet．与英德睑虎G．yingdeensis以及凭祥睑虎G．luii与蛛纹睑虎G．araneus两两种间的亲缘关系较近；且分布区相邻的Goniurosaurus indet．与G．yingdeensis之间的遗传分化（遗传距离＝0.044-0.046）相似于分类地位已经明确的G．luii与G．araneus种间的遗传差异（遗传距离＝0．051）．结合形态学研究结果，认为Goniurosaurus indet．和G．yingdeensis之间已发生显著的遗传差异，前者可能为睑虎属新种．同时，系统发育分析表明海南睑虎G．hainanensis与睑虎属其它物种之间的遗传分化较大，系统发育地位最为特殊．系统发育分析进一步表明具4条背部横纹是祖征，具5条背部横纹为衍征．该文首次提出宽阔的河流可能是造成睑虎种群分化和新种形成的重要地理屏障．这一“河流隔离”观点对全球睑虎属物种的亲缘地理分布格局的解释具有重要的理论意义．
료해아국검호속Goniurosaurus （ Squamata：Sauria：Eublepharidae ）충간적친연관계이급엄동신기록적검호속미정충Goniurosaurus indet．적유효성，대검호속10개충적선립체DNA기인편단비교화유전변이분석．획득12S rRNA （385 bp）화16S rRNA （477 bp）총장도위862 bp적연합서렬，공유핵감산변이위점388개，간약신식위점269개．선취대벽호Gekko gecko위외류군，채용패협사연역법（BI）、최대사연법（ML）、최대간약법（MP）、린접법（NJ）대량개기인연합서렬적분석균현시상동결과，즉엄동검호속미정충Goniurosaurus indet．여영덕검호G．yingdeensis이급빙상검호G．luii여주문검호G．araneus량량충간적친연관계교근；차분포구상린적Goniurosaurus indet．여G．yingdeensis지간적유전분화（유전거리＝0.044-0.046）상사우분류지위이경명학적G．luii여G．araneus충간적유전차이（유전거리＝0．051）．결합형태학연구결과，인위Goniurosaurus indet．화G．yingdeensis지간이발생현저적유전차이，전자가능위검호속신충．동시，계통발육분석표명해남검호G．hainanensis여검호속기타물충지간적유전분화교대，계통발육지위최위특수．계통발육분석진일보표명구4조배부횡문시조정，구5조배부횡문위연정．해문수차제출관활적하류가능시조성검호충군분화화신충형성적중요지리병장．저일“하류격리”관점대전구검호속물충적친연지리분포격국적해석구유중요적이론의의．
We studied the genetic differentiation of Goniurosaurus indet.from northwestern Guangdong Province , China, and its phylogenetic relationships with other Chinese species of the Goniurosaurus genus, using partial se-quences of the mitochondrial 12S rRNA and 16S rRNA genes of 10 species in total and Gekko gecko as the out-group .A total sequence of 862 nucleotides with 12 S rRNA ( 385 bp ) and 16 S rRNA ( 477 bp ) genes was ana-lyzed;388 variable sites and 123 parsimoniously informative sites were detected in combination of the two genes . Analyses of Bayesian inference ( BI) , maximum likelihood ( ML) , maximum parsimony ( MP) , and neighbor-join-ing ( NJ) all showed similar results: a close phylogenetic relationship between Goniurosaurus indet.and G.ying-deensis (genetic distance =0.044-0.046), comparable to that between accepted known species G.araneus and G.luii (genetic distance =0.051).Combined with morphological differences , our results support the notion that there is significant genetic differentiation between Goniurosaurus indet.and G.yingdeensis even though the two forms are separated only by a river;thus, Goniurosaurus indet.may be a new species.The phylogenetic analyses also in-dicated the uniqueness of G.hainanensis with the greatest genetic distance from others , suggesting that the character state of four body bands including nuchal loop is plesiomorphic as compared to that of five body bands .We hypoth-esized for the first time that rivers act as isolating barrier for population differentiation and speciation among conti -nental Goniurosaurus species .