CAJ | 학술논문

目的：观察旋毛虫的低温耐受性和感染性。方法20只雌性昆明小鼠被随机分为对照组和实验组，实验组根据温度不同分3组，共4组，每组5只。对照组每鼠经口感染150条肌幼虫。实验组①组（低温1 h组）：肉样置于-18℃、1 h；②组（低温4 h组）：肉样置于-18℃、4 h；③组（低温24 h组）：肉样置于-18℃、24 h；经低温处理的3组鼠肉取出后，室温下（16℃）彻底融化，磁力搅拌法（消化2 h ）收集幼虫。然后分别人工灌胃小鼠，每鼠经口感染150条肌幼虫。所有小鼠感染后28 d剖杀，取膈肌压片镜检，并用磁力搅拌法收集肌幼虫、计数。结果对照组、低温1h组、低温4h组和低温24h组的肌幼虫均数分别为：54904．0、23976．0、3196．0、0；低温1h组和低温4h组2组的肌幼虫均数显著低于对照组（P＜00．1）；低温4h组的肌幼虫均数低于低温1h组（P ＜00．5）。结论低温（18℃）对旋毛虫具有杀伤作用，旋毛虫囊内幼虫的活力和感染性与冷冻时间和肉样厚度均有直接关系。
목적：관찰선모충적저온내수성화감염성。방법20지자성곤명소서피수궤분위대조조화실험조，실험조근거온도불동분3조，공4조，매조5지。대조조매서경구감염150조기유충。실험조①조（저온1 h조）：육양치우-18℃、1 h；②조（저온4 h조）：육양치우-18℃、4 h；③조（저온24 h조）：육양치우-18℃、24 h；경저온처리적3조서육취출후，실온하（16℃）철저융화，자력교반법（소화2 h ）수집유충。연후분별인공관위소서，매서경구감염150조기유충。소유소서감염후28 d부살，취격기압편경검，병용자력교반법수집기유충、계수。결과대조조、저온1h조、저온4h조화저온24h조적기유충균수분별위：54904．0、23976．0、3196．0、0；저온1h조화저온4h조2조적기유충균수현저저우대조조（P＜00．1）；저온4h조적기유충균수저우저온1h조（P ＜00．5）。결론저온（18℃）대선모충구유살상작용，선모충낭내유충적활력화감염성여냉동시간화육양후도균유직접관계。
Objective To observe the infectivity and freeze resistance of Trichinella spiralis . Methods Twenty female Kunming mice were randomly divided into one control group and three test groups , so 4 groups ( with 5 mice per group) in total . For the control group each mouse was inoculated orally with 150 muscle larvae of T .spiralis . Test Group one?s samples were stored at -18 ℃ for 1h;test group two?s samples were stored at -18℃ for 4 h;test group three?s samples were stored at -18℃ for 24 h . All samples were taken out and thawed;muscle larvae of T . spiralis were collected by the magnetic stirrer method . Then , each mouse was orally inoculated with 150 disposed muscle larval of T .spiralis . All infected mice were sacrificed on the 28 th day after infectio , and muscle larval of T .spiralis were examined respectively by diaphragma compression method and digestion method for all infected mice .Results The average number of muscle larvae stored at -18℃ for 1h and 4h were 2 397 .60 and 319 .60 , respectively . These two groups?averages were obviously lower than that of the muscle larvae control group (P < 0 .01 ) .Group two (4 h)?s averages were lower than that of the group one ( 1 h) (P < 0 .05 ) .Conclusion Freezing does the vital harm to the muscle larval of T . spiralis . The activity and infectivity of muscle larvae are dependent on both freezing periods and muscle depth .