CAJ | 학술논문

目的：观察皂角刺水煎液的体内外抗肿瘤作用。方法体内实验：建立小鼠背部皮下移植性实体肿瘤H22荷瘤动物模型，灌胃给药后测定肿瘤体积、质量及免疫器官指数；体外实验：用M T T法测定皂角刺对小鼠lew is肺癌细胞的影响。结果体内实验：在本实验剂量下（03．mL／g），与模型对照组比较，皂角刺组肿瘤生长减缓，抑瘤率为383．7％，胸腺指数、脾脏指数均增高；体外实验：实验所用浓度的皂角刺水煎液呈剂量依赖性抑制小鼠lew is肺癌细胞生长。结论皂角刺有一定抗肿瘤作用，其机制可能与细胞毒和提高机体免疫作用有关。
목적：관찰조각자수전액적체내외항종류작용。방법체내실험：건립소서배부피하이식성실체종류H22하류동물모형，관위급약후측정종류체적、질량급면역기관지수；체외실험：용M T T법측정조각자대소서lew is폐암세포적영향。결과체내실험：재본실험제량하（03．mL／g），여모형대조조비교，조각자조종류생장감완，억류솔위383．7％，흉선지수、비장지수균증고；체외실험：실험소용농도적조각자수전액정제량의뢰성억제소서lew is폐암세포생장。결론조각자유일정항종류작용，기궤제가능여세포독화제고궤체면역작용유관。
Objective To observe antitumor effects of spina gleditsiae water extract in vitro and in vivo . Methods In vivo :the subcutaneous model of H22 liver cancer was established to observe antitumor by measuring the volume , weight of in situ tumor and immune organs index after ig of spina gleditsiae water extract . In vitro :MTT assay is used to test antitumor effects of spina gleditsiae water extract on lewis lung cancer cells . Results In vivo :compared with model control group , the tumor size in spina gleditsiae group was smaller , the inhibitionory rate was 38 .37% , at the same time , the thymus index and spleen index increased . In vitro :spina gleditsiae water extract inhibited proliferation in a concentration dependent matter . Conclusion Spina gleditsiae have antitumor activity , its mechanism might be related to cytotoxity and immune modulation .