CAJ | 학술논문

目的：研究升降二丹对小鼠体表肿瘤的治疗作用。方法建立小鼠肝癌H22、肺癌lew is皮下移植肿瘤模型和小鼠乳腺癌模型，通过小鼠存活期、肿瘤体积、肿瘤抑制率、脏器指数等考察升降二丹对小鼠体表肿瘤生长的影响；用MTT法观察升降二丹对lewis肿瘤细胞增殖的影响；ELISA法检测瘤组织TNF-α及CEA含量，初步探讨升降二丹的抗肿瘤机制。结果升降二丹能使小鼠体表肿瘤逐渐缩小，延长荷瘤小鼠生存时间，阻止小鼠肿瘤自发转移，增加肿瘤组织TNF-α，减少肿瘤组织CEA ；体外实验，升降二丹在高浓度可抑制肿瘤细胞的增长。结论升降二丹有明显的抗体表肿瘤作用，其机制可能与增加肿瘤组织TNF-α含量和减少肿瘤组织CEA含量有关。
목적：연구승강이단대소서체표종류적치료작용。방법건립소서간암H22、폐암lew is피하이식종류모형화소서유선암모형，통과소서존활기、종류체적、종류억제솔、장기지수등고찰승강이단대소서체표종류생장적영향；용MTT법관찰승강이단대lewis종류세포증식적영향；ELISA법검측류조직TNF-α급CEA함량，초보탐토승강이단적항종류궤제。결과승강이단능사소서체표종류축점축소，연장하류소서생존시간，조지소서종류자발전이，증가종류조직TNF-α，감소종류조직CEA ；체외실험，승강이단재고농도가억제종류세포적증장。결론승강이단유명현적항체표종류작용，기궤제가능여증가종류조직TNF-α함량화감소종류조직CEA함량유관。
Objective To study the anti tumor effects of hydrargyrum oxydatum crudum bottom with hydrargyrum chloratum compositum .Methods The transplanted tumor models of mouse H22 , 4T1 and lewis were established and the antitumor activity of hydrargyrum oxydatum crudum bottom with hydrargyrum chloratum compositum was observed by the survival tumor size , tumor inhibition rate , organ index . The effects of hydrargyrum oxydatum crudum bottom with hydrargyrum chloratum compositum lewis tumor cells were examined by MTT method in vitro . The mechanism by which hydrargyrum oxydatum crudum bottom with hydrargyrum chloratum compositum showed the anti-tumor effects was explored by examining TNF-αand CEA levels in tumor tissue using ELISA . Results Hydrargyrum oxydatum crudum bottom with hydrargyrum chloratum compositum reduced tumor size , prolonged mouse survival time , prevented tumor metastasis , increased TNF-αlevel in tumor , and decreased CEA level in tumor . In vitro , hydrargyrum oxydatum crudum bottom with hydrargyrum chloratum compositum at high concentration can inhibit tumor cell grow th . Conclusion Hydrargyrum oxydatum crudum bottom with hydrargyrum chloratum compositum has anti-tumor effect on skin tumor ,and its mechanism might be related to increasing TNF-αand reducing CEA in tumor cells .