CAJ | 학술논문

目的以小鼠为动物模型，通过检测布氏菌活疫苗免疫小鼠产生的体液免疫、细胞免疫应答及保护力，对其进行免疫学评价。 　　方法将30只小鼠随机分为3组，皮下免疫布氏活疫苗，免疫4周后分离血清及脾脏淋巴细胞。ELISA法检测免疫动物血清中总 IgG 抗体，抗体亚类 IgG1及 IgG2a；ELISPOT 法检测分泌 IFN-γ、IL-4的脾脏淋巴细胞数目，以及用 ELISA 方法检测体外再刺激后小鼠脾细胞分泌IFN-γ、IL-4的细胞因子水平；用羊布氏菌弱毒株 M5攻击免疫动物，通过脾脏细菌计数评价布氏活疫苗的免疫保护作用。 　　结果用 ELISA 法检测到免疫布氏活苗的小鼠体内有特异性抗体产生；ELISPOT 可检测到较高的分泌 IFN-γ脾脏淋巴细胞数目，ELISA 方法可检测到较高的 IFN-γ水平，表明布氏活疫苗主要诱导 Th1型免疫应答；通过布氏活疫苗保护力分析，表明该疫苗具有较好的免疫保护效果。 　　结论布氏疫苗采用注射免疫途径是可行的，免疫后能获得较好的免疫效果。
목적이소서위동물모형，통과검측포씨균활역묘면역소서산생적체액면역、세포면역응답급보호력，대기진행면역학평개。 　　방법장30지소서수궤분위3조，피하면역포씨활역묘，면역4주후분리혈청급비장림파세포。ELISA법검측면역동물혈청중총 IgG 항체，항체아류 IgG1급 IgG2a；ELISPOT 법검측분비 IFN-γ、IL-4적비장림파세포수목，이급용 ELISA 방법검측체외재자격후소서비세포분비IFN-γ、IL-4적세포인자수평；용양포씨균약독주 M5공격면역동물，통과비장세균계수평개포씨활역묘적면역보호작용。 　　결과용 ELISA 법검측도면역포씨활묘적소서체내유특이성항체산생；ELISPOT 가검측도교고적분비 IFN-γ비장림파세포수목，ELISA 방법가검측도교고적 IFN-γ수평，표명포씨활역묘주요유도 Th1형면역응답；통과포씨활역묘보호력분석，표명해역묘구유교호적면역보호효과。 　　결론포씨역묘채용주사면역도경시가행적，면역후능획득교호적면역효과。
Objective To evaluate the immune response of live Brucella vaccine for intradermal injection, which detect humoral immunity and cellular immune responses in mice. Methods Mice were randomly divided into 3 groups with intradermal immune live Brucella vaccine, After 4 weeks, blood was sampled and spleen lymphocytes were separated. Then serum ELISA was used to detect the immune animal total IgG antibody, IgG1, IgG2a The number of spleen lymphocytes with secretion of IFN-γ, IL-4 was examined by ELISPOT. ELISA method was adopted to detect the level of cytokines IFN-γ, IL-4 from spleen cells after restimulation in vitro. After Brucella melitensis M5 infected animals, the spleen were isolated in sterile. The acquired immune protection of immune Brucella vaccine was evaluated through the bacteria count of spleen. Results The ELISA results showed that there exerted specific antibodies in mice infected by immune live Brucella vaccine. The number of spleen lymphocytes with secretion of INF-γwas increased through ELISPOT assay. The level of IFN-γwas higher than the control after detection of ELISA method. From this, live Brucella vaccine for intradermal injection mainly induced type TH1 immune response. The results from analysis of protection force of live Brucella vaccine demonstrated that the vaccine produced strong immune protection. Conclusion The method of live Brucella vaccine for intradermal injection is feasible, which can get intensively immunogenicity.