CAJ | 학술논문

已有研究显示同为芽孢杆菌其抑菌作用机制存在着显著不同，本文通过16S rRNA序列和gyrB基因序列等方法鉴定了8株芽孢杆菌，分析了其生物活性。结果表明，8株芽孢杆菌均为解淀粉芽孢杆菌Bacillus amyloliquefaciens；表型及生物活性分析显示，8株解淀粉芽孢杆菌在菌落形态、菌体大小、芽孢形成等方面存在差异；菌株JT261和T455具有较强的分泌蛋白酶能力、菌株JT84具有较强的分泌嗜铁素能力；菌株JT81和T429形成生物膜能力较强；菌株JT81具有较强的抑菌活性，菌株T455分泌物质具有较强的抑菌活性；本研究为从分子遗传学角度解析同为解淀粉芽孢杆菌生物活性差异提供了研究基础。
이유연구현시동위아포간균기억균작용궤제존재착현저불동，본문통과16S rRNA서렬화gyrB기인서렬등방법감정료8주아포간균，분석료기생물활성。결과표명，8주아포간균균위해정분아포간균Bacillus amyloliquefaciens；표형급생물활성분석현시，8주해정분아포간균재균락형태、균체대소、아포형성등방면존재차이；균주JT261화T455구유교강적분비단백매능력、균주JT84구유교강적분비기철소능력；균주JT81화T429형성생물막능력교강；균주JT81구유교강적억균활성，균주T455분비물질구유교강적억균활성；본연구위종분자유전학각도해석동위해정분아포간균생물활성차이제공료연구기출。
Even the same Bacillus species are significantly different in mode of antimicrobial action. To explain the difference of the Bacillus species in antimicrobial activity, we identified eight Bacillus isolates through analysis of 16S rRNA and gyrB gene sequences. Results showed that all of the eight isolates belonged to B. amyloliquefaciens, although they were different in colonial morphology, thallus sizes and sporulation. The differences also exhibited in biological activities. Results showed that strain JT261 and T455 exhibited stronger abilities of protease, strain JT84 manifested better ability of siderophores;strain JT81 and T429 had strong ability in biofilm formation. These results provide information for understanding the difference of isolates in B. amyloliquefaciens in biological activities at molecular level.