CAJ | 학술논문

Thga1是哈茨木霉Th-33的1种I型G蛋白α亚基基因（GenBank JN874387）。本文针对前期研究获得的2株基因Thga1敲除突变株ΔThga11-1和ΔThga110-1，通过平皿对峙培养分析其对立枯丝核菌、辣椒疫霉菌和尖孢镰刀菌的拮抗作用，研究了突变株产生的挥发性代谢物和非挥发性代谢物对上述3种病原菌生长的影响和对立枯丝核菌的重寄生作用的光学显微镜观察。结果表明，与野生型Th-33相比，基因敲除突变株ΔThga11-1和ΔThga110-1对病原菌的拮抗作用显著下降，不能对3种病原菌菌落进行覆盖和寄生；突变株产生的挥发性代谢产物对病原菌生长的抑制率显著低于野生菌 Th-33，2株突变株对立枯丝核菌、辣椒疫霉和尖孢镰刀菌生长的抑制率分别降低了50%～57%、43%～58%和58%～63%，且突变株之间差异不显著；突变株产生的非挥发性代谢产物对病原菌生长的抑制作用中，除突变株ΔThga11-1对立枯丝核菌的抑制作用与野生菌相比差异不显著外，均显著低于野生菌Th-33，突变株ΔThga11-1和ΔThga110-1对3种病原菌的抑制率分别降低了8%～25%、23%～56%和38%～50%。显微观察发现，野生菌Th-33菌丝能够缠绕、附着、穿透和裂解立枯丝核菌菌丝，但突变株菌丝对立枯丝核菌菌丝没有发现类似作用。上述结果表明，Thga1基因影响了哈茨木霉菌的拮抗、次级代谢和重寄生等作用。
Thga1시합자목매Th-33적1충I형G단백α아기기인（GenBank JN874387）。본문침대전기연구획득적2주기인Thga1고제돌변주ΔThga11-1화ΔThga110-1，통과평명대치배양분석기대립고사핵균、랄초역매균화첨포렴도균적길항작용，연구료돌변주산생적휘발성대사물화비휘발성대사물대상술3충병원균생장적영향화대립고사핵균적중기생작용적광학현미경관찰。결과표명，여야생형Th-33상비，기인고제돌변주ΔThga11-1화ΔThga110-1대병원균적길항작용현저하강，불능대3충병원균균락진행복개화기생；돌변주산생적휘발성대사산물대병원균생장적억제솔현저저우야생균 Th-33，2주돌변주대립고사핵균、랄초역매화첨포렴도균생장적억제솔분별강저료50%～57%、43%～58%화58%～63%，차돌변주지간차이불현저；돌변주산생적비휘발성대사산물대병원균생장적억제작용중，제돌변주ΔThga11-1대립고사핵균적억제작용여야생균상비차이불현저외，균현저저우야생균Th-33，돌변주ΔThga11-1화ΔThga110-1대3충병원균적억제솔분별강저료8%～25%、23%～56%화38%～50%。현미관찰발현，야생균Th-33균사능구전요、부착、천투화렬해립고사핵균균사，단돌변주균사대립고사핵균균사몰유발현유사작용。상술결과표명，Thga1기인영향료합자목매균적길항、차급대사화중기생등작용。
Trichoderma harzianum Th-33 antagonize against a variety of plant pathogenic fungi. Thga1 (GenBank JN874387) of strain Th-33 codes a type I GTP binding protein α subunit. Two Thga1 gene knock-out mutantsΔThgal 1-1 and ΔThgal 10-1 had been constructed in our previous studies. The antagonistic effects of the two mutants against three plant pathogens including Rhizoctonia solani, Phytophthora capsici and Fusarium oxysporum were studied in this paper. The two mutants could not cover and mycoparasitize the colonies of tested pathogens and the antagonisms reduced significantly comparing to wild-type Th-33 when confronted with tested plant pathogens. The inhibitory ratios of volatile metabolites fromΔThgal 1-1 andΔThgal 10-1 to the tested plant pathogens reduced 50%-57%to R. solani, 43%-58%to P. capsici and 58%-63%to F. oxysporum comparing to that of wild-type Th-33 respectively, and there was no significant difference between the two mutants. Inhibition ratios of non-volatile metabolites fromΔThgal 1-1 andΔThgal 10-1 to the three tested plant pathogens reduced 8%-25%to R. solani, 23%-56%to P. capsici and 38%-50%to F. oxysporum, respectively. The parasitism of wild-type Th-33 against R. solani such as hyphae winding, adhering, penetration and leading to hyphae disruption were observed under microscope, but we have not observed the above phenomena resulting from the two mutants. The results showed that Thga1 influenced antagonism, secondary metabolism and mycoparasitism of T. harzianum.