CAJ | 학술논문

从自然死亡黄曲条跳甲幼虫体中分离出1株对黄曲条跳甲幼虫和成虫有较高致病性菌株PS-1。通过病原菌的形态观察、Biolog系统鉴定以及16S rDNA序列测定分析，确定菌株PS-1隶属于肠杆菌科、沙雷氏菌属的粘质沙雷氏菌Serratia marcescens族群。用菌株PS-1浓度为3×104、3×106和3×108 cfu/mL的菌悬液浸泡鲜萝卜块饲喂黄曲条跳甲幼虫24 h，4 d后的校正死亡率分别为36.1%、63.9%和75.0%；2、3和4 d的LC50分别是7.98×106、4.27×106和8.02×105 cfu/mL。用浓度1×108 cfu/mL的菌悬液处理菜心叶片饲喂黄曲条跳甲成虫，12 d的校正死亡率为23.25%。用浓度1×105、1×107和1×109 cfu/mL的菌悬液浸渍黄曲条跳甲卵，其孵化率与对照无显著差异。结果表明，粘质沙雷氏菌 PS-1菌株对黄曲条跳甲成虫和幼虫有较强的毒力，在黄曲条跳甲的生物防治方面具有一定的应用前景。
종자연사망황곡조도갑유충체중분리출1주대황곡조도갑유충화성충유교고치병성균주PS-1。통과병원균적형태관찰、Biolog계통감정이급16S rDNA서렬측정분석，학정균주PS-1대속우장간균과、사뢰씨균속적점질사뢰씨균Serratia marcescens족군。용균주PS-1농도위3×104、3×106화3×108 cfu/mL적균현액침포선라복괴사위황곡조도갑유충24 h，4 d후적교정사망솔분별위36.1%、63.9%화75.0%；2、3화4 d적LC50분별시7.98×106、4.27×106화8.02×105 cfu/mL。용농도1×108 cfu/mL적균현액처리채심협편사위황곡조도갑성충，12 d적교정사망솔위23.25%。용농도1×105、1×107화1×109 cfu/mL적균현액침지황곡조도갑란，기부화솔여대조무현저차이。결과표명，점질사뢰씨균 PS-1균주대황곡조도갑성충화유충유교강적독력，재황곡조도갑적생물방치방면구유일정적응용전경。
A virulent strain PS-1 with high infectivity was isolated from the natural death larvae of Phyllotreta striolata. The strain PS-1 was characterized as Serratia marcescens based on its morphological, cultural, biolog identification system and 16S rDNA sequences analysis. The larvae of P. striolata were fed with turnip cubes which had been dipped in suspension culture of different concentrations (3×104, 3×106 and 3×108 cfu/mL) of S. marcescens PS-1 for 24 h. The corrected mortality rates were 36.1%, 63.9% and 75.0% at 4 d post-treatment, respectively. The LC50 was 7.98×106, 4.27×106 and 8.02×105 cfu/mL at 2, 3 and 4 d. The corrected mortality rate of P. striolata larvae fed with cabbage leaves which had been dipped in suspension culture of S. marcescens PS-1 under the concentrations of 1×108 cfu/mL was 23.25%at 12 d post-treatment. When the eggs of P. striolata were dipped in suspension culture of different concentrations (1×105, 1×107and 1×109 cfu/mL) of S. marcescens PS-1, there was no significant difference in the hatching rate between the control and treatments. The results suggest that S. marcescens PS-1 which has a strong virulence against the larvae and adults of P. striolata might be a good candidate as a potential microbial agent for biological control.