CAJ | 학술논문

TATA结合蛋白（TATA-binding protein，TBP）及其相关因子是转录起始复合物TFIID的重要组分，能识别 TATA元件并与之特异性结合，进而指导 RNA聚合酶和其它转录因子有序装配，形成稳定的转录起始复合物。本文采用RT-PCR和RACE技术克隆获得了菜蛾盘绒茧蜂Cotesia vestalis畸形细胞TATA结合蛋白（CvT-TBP）基因的cDNA序列（GenBank No. JX984954），序列全长6105 bp，开放阅读框5784 bp，编码1927个氨基酸，预测分子量213.8 kDa，理论等电点6.82。氨基酸序列分析表明，CvT-TBP与其他膜翅目昆虫的TBP有很高的同源性，其中与切叶蜂Megachile rotundata、小蜜蜂Apis florea和佛罗里达弓背蚁Camponotus floridanus的相似性均为72%。本研究克隆得到的畸形细胞TATA box结合蛋白cDNA序列，为进一步研究该基因功能以及畸形细胞的作用机理提供了基础数据。
TATA결합단백（TATA-binding protein，TBP）급기상관인자시전록기시복합물TFIID적중요조분，능식별 TATA원건병여지특이성결합，진이지도 RNA취합매화기타전록인자유서장배，형성은정적전록기시복합물。본문채용RT-PCR화RACE기술극륭획득료채아반융충봉Cotesia vestalis기형세포TATA결합단백（CvT-TBP）기인적cDNA서렬（GenBank No. JX984954），서렬전장6105 bp，개방열독광5784 bp，편마1927개안기산，예측분자량213.8 kDa，이론등전점6.82。안기산서렬분석표명，CvT-TBP여기타막시목곤충적TBP유흔고적동원성，기중여절협봉Megachile rotundata、소밀봉Apis florea화불라리체궁배의Camponotus floridanus적상사성균위72%。본연구극륭득도적기형세포TATA box결합단백cDNA서렬，위진일보연구해기인공능이급기형세포적작용궤리제공료기출수거。
The TATA-binding protein (TBP) and other associated factors are the central elements of the transcription factor, TFIID. The TATA-binding protein recognizes the TATA sequence and specifically binds to it, which recruits RNA polymerase and other transcription factors to form a stable transcription initiation complex. Here we cloned a TATA binding protein gene of Cotesia vestalis teratocyte (CvT-TBP) (GenBank No. JX984954) using RT-PCR and RACE methods. The full length of this cDNA fragment is 6105 bp, containing an open reading frame of 5784 bp which encodes 1927 amino acid residues with a predicted molecular weight of 213.8 kDa and isoelectric point of 6.82. Homology analysis using BlastP showed that the amino acid sequence of CvT-TBP had a high similarity with those of TBPs from other insects, sharing 72% identity with those of Megachile rotundata, Apis florea and Camponotus floridanus. The results provide a basis for further study of the functional mechanism of teratocytes.