CAJ | 학술논문

为充分发掘新疆盐环境中生防活性放线菌，以棉花黄萎菌为靶标，从805株新疆盐环境放线菌中筛选获得14株拮抗菌，其中菌株TRM42561抑菌活性最强，其发酵液对棉花黄萎菌的抑菌圈直径达26.33 mm，且对6种常见植物病原真菌和4种细菌均有较好的抑制效果，抗菌谱广。室内抑菌活性表明，拮抗菌TRM42561发酵液对棉花黄萎菌菌丝生长抑制率为83.75%，孢子萌发抑制率为68.75%。田间试验结果表明，拮抗菌TRM42561对棉花黄萎病的田间防治效果显著，在3次施药后的第15和30 d，其防治效果分别达到33.06%和41.65%。通过菌株形态特征、培养特性、生理特性以及16S rDNA序列分析，确定拮抗菌TRM42561为娄彻氏链霉菌Streptomyces rochei。
위충분발굴신강염배경중생방활성방선균，이면화황위균위파표，종805주신강염배경방선균중사선획득14주길항균，기중균주TRM42561억균활성최강，기발효액대면화황위균적억균권직경체26.33 mm，차대6충상견식물병원진균화4충세균균유교호적억제효과，항균보엄。실내억균활성표명，길항균TRM42561발효액대면화황위균균사생장억제솔위83.75%，포자맹발억제솔위68.75%。전간시험결과표명，길항균TRM42561대면화황위병적전간방치효과현저，재3차시약후적제15화30 d，기방치효과분별체도33.06%화41.65%。통과균주형태특정、배양특성、생리특성이급16S rDNA서렬분석，학정길항균TRM42561위루철씨련매균Streptomyces rochei。
In order to discovery antagonistic actinomycetes from saline environments in Xinjiang, we tested the antifungal activities of 805 strains of halophilic actinomycetes against Verticillium dahliae. Among the 14 strains showing strong antagonistic activity, actinomycete TRM42561 showed the strongest mycelium inhibiting activity against the target pathogen with the inhibition diameter of 26.33 mm. Besides, TRM42561 exhibited broad antifungal and antibacterial activities on six fungi and four bacteria. Moreover, both the mycelium growth and the spore germination of V. dahliae were inhibited by the fermentaion broth of TRM42561, and the inhibiting rate were 83.75%and 68.75%, respectively. Field experiment showed that the fermentation broth of TRM42561 exhibited a protective efficacy of 33.06%and 41.65%against cotton Verticillium wilt in the fifteenth and thirtieth days after the third root-drenching. Based on morphological, cultural, physiological characteristics and 16S rDNA sequences analysis, we identified TRM42561 as Streptomyces rochei.