CAJ | 학술논문

目的：考察菊黄清热散的抗菌、解热、镇痛、抗炎和免疫作用。方法采用倍比稀释法检测菊黄清热散体外抗病原菌作用；给予BABL/C小鼠腹腔接种肺炎链球菌观察体内抗菌作用；考察家兔内毒素发热模型的解热作用；以小鼠热板法和扭体法研究镇痛作用；以大鼠足趾肿胀模型及小鼠腹腔毛细血管通透性模型研究抗炎作用；以小鼠碳粒廓清法研究免疫调节作用。结果菊黄清热散在体外有一定的抗菌作用，能降低体内感染小鼠的死亡率；菊黄清热散中、低剂量组在给药后2～3 h发热家兔体温显著降低（P＜0．05），高剂量组2～4 h时显著降低（P＜0．05）；菊黄清热散各剂量组在给药后1．5、2、3 h时小鼠痛阈值显著升高（P＜0．05），且能显著降低腹腔注射后小鼠的扭体次数（P＜0．05）；菊黄清热散各剂量组给药后1、2、3、4、5 h大鼠足趾肿胀率显著降低（P＜0．05），可降低模型小鼠急性炎症早期的腹腔毛细血管通透性（P＜0．05）；在碳粒廓清法免疫调节作用中，菊黄清热散高、中剂量组测得廓清指数K值明显提高，吞噬指数α值也有一定程度的增加（P＜0．05）。结论实验提示菊黄清热散具有抗菌、解热、镇痛、抗炎和增强免疫的作用。
목적：고찰국황청열산적항균、해열、진통、항염화면역작용。방법채용배비희석법검측국황청열산체외항병원균작용；급여BABL/C소서복강접충폐염련구균관찰체내항균작용；고찰가토내독소발열모형적해열작용；이소서열판법화뉴체법연구진통작용；이대서족지종창모형급소서복강모세혈관통투성모형연구항염작용；이소서탄립곽청법연구면역조절작용。결과국황청열산재체외유일정적항균작용，능강저체내감염소서적사망솔；국황청열산중、저제량조재급약후2～3 h발열가토체온현저강저（P＜0．05），고제량조2～4 h시현저강저（P＜0．05）；국황청열산각제량조재급약후1．5、2、3 h시소서통역치현저승고（P＜0．05），차능현저강저복강주사후소서적뉴체차수（P＜0．05）；국황청열산각제량조급약후1、2、3、4、5 h대서족지종창솔현저강저（P＜0．05），가강저모형소서급성염증조기적복강모세혈관통투성（P＜0．05）；재탄립곽청법면역조절작용중，국황청열산고、중제량조측득곽청지수K치명현제고，탄서지수α치야유일정정도적증가（P＜0．05）。결론실험제시국황청열산구유항균、해열、진통、항염화증강면역적작용。
Objective To investigate the efficacy on antibacterial , antipyretic, analgesic, anti-inflammatory and immune reg-ulation of Juhuang Qingre San .Methods Efficacy on antibacterial was evaluated by the method of doubling dilution ,vivo antibacterial effect was observed by inoculating BABL/C mice with Streptococcus pneumonia ,efficacy on antipyretic was investigated by using endo-toxin fever model ,analgesic function was assessed using mice hot plate and torsion analgesic method ,effect of anti inflammatory was as-sessed with rat paw edema model and mice Peritoneal capillary permeability model , the role of regulating immune system was evaluated by mice carbon clearance test .Results Juhuang Qingre San can decrease the death rate of mice in antibacterial test in vitro or vivo,in low and mid dose group of Juhuang Qingre San , heating temperature of rabbits decreased significantly 2-3 h after administration ( P<0.05), and high dosage group decreased significantly 2-4 h after administration (P<0.05),the mice pain threshold increased signif-icantly (P<0.05) after the administration of 1.5, 2, 3 h in every group of Juhuang Qingre San was , and can significantly reduce the number of mice writhing after intraperitoneal injection (P<0.05),rat paw swelling rate decreased significantly 1, 2, 3, 4, 5 h after administration in each dosage group of Juhuang Qingre San (P<0.05), peritoneal capillary permeability decreased in the acute in-flammation model of mice (P<0.05), in carbon particle clearance test of immune regulation , K value of high and medium dose group of Juhuang Qingre San was increased , while the value of alpha increased as well ( P<0 .05 ) .Conclusion The results suggested that Juhuang Qingre San has efficacy of antibacterial , antipyretic, analgesic, anti-inflammatory and immune enhancing .