CAJ | 학술논문

目的：建立原代心肌细胞缺氧-复氧模型，并对麝香保心丸及其20种血中活性成分进行抗缺氧-复氧损伤的活性筛选。方法取新生（1～3 d）SD乳鼠的心脏，建立原代心肌细胞的缺氧-复氧损伤模型，并采用四甲基偶氮唑盐（MTT）比色法进行麝香保心丸及其入血成分的活性筛选。结果麝香保心丸在50μg/ml浓度下具有较好的保护原代心肌细胞缺氧-复氧损伤的作用；人参皂苷Rb1、人参皂苷Rb2、蟾毒灵和麝香酮具有较好的保护原代心肌细胞缺氧-复氧损伤的作用。结论麝香保心丸具有抗心肌细胞缺氧-复氧损伤的作用，人参皂苷Rb1、人参皂苷Rb2、蟾毒灵和麝香酮是其主要的有效成分。该研究为麝香保心丸深入的药效和机制研究奠定了基础。
목적：건립원대심기세포결양-복양모형，병대사향보심환급기20충혈중활성성분진행항결양-복양손상적활성사선。방법취신생（1～3 d）SD유서적심장，건립원대심기세포적결양-복양손상모형，병채용사갑기우담서염（MTT）비색법진행사향보심환급기입혈성분적활성사선。결과사향보심환재50μg/ml농도하구유교호적보호원대심기세포결양-복양손상적작용；인삼조감Rb1、인삼조감Rb2、섬독령화사향동구유교호적보호원대심기세포결양-복양손상적작용。결론사향보심환구유항심기세포결양-복양손상적작용，인삼조감Rb1、인삼조감Rb2、섬독령화사향동시기주요적유효성분。해연구위사향보심환심입적약효화궤제연구전정료기출。
Objective To build hypoxia/reoxygenation injury model in cultured neonatal rat cardiomyocyte and screen active components from Shexiang Baoxin Pill ( SBP) absorbed in blood against hypoxia/reoxygenation injury .Methods Cardiomyocytes were isolated and purified from hearts of neonatal Sprague Dawley rats (1~3 days old) and were used to build hypoxia/reoxygenation injury model.The components of SBP absorbed in blood were screened by methyl thiazolil tetracolium (MTT) colorimetic method.Results SBP showed significant protective effect against cardiomyocytes hypoxia /reoxygenation injury atthe concentration of 50 μg/ml.Ginsen-oside Rb1, Rb2, bufalin and muscone of twenty components from SBP absorbed in blood also possessed significant protective effect a -gainst cardiomyocytes hypoxia/reoxygenation injury .Conclusion SBP have the protective activity against cardiomyocytes hypoxia /reoxygenation injury , and ginsenoside Rb1, Rb2, bufalin, muscone are the main active components of SBP .This experiment offered basis for further pharmacodynamics and mechanism study of SBP .