CAJ | 학술논문

目的：探讨右心室心尖部起搏对Beagle犬心室肌电重构及细胞外信号调节激酶（ERK）1/2磷酸化水平的影响。方法成年雄性Beagle犬12只，年龄3~4年，体重20~24kg，随机分为对照组（n=6）和起搏组（n=6），均植入DDD起搏器后程控至关闭状态，起搏组术后1周时开启起搏器并使心室起搏最大化。于术前及术后1周、4周关闭起搏器后行心电图及超声心动描记术检查，术后4周时处死动物，观察心肌病理结果，采用免疫印迹法检测犬右心室心肌总ERK1/2及其磷酸化水平的变化。结果两组心室肌病理及超声心动描记术、心率、QRS时间、总ERK1/2的表达差异均无统计学意义（均P＞0.05）；与对照组相比，术后4周时起搏组Tp- e间期、Q- T间期均增大，p- ERK增多（均P＜0.05）。结论右心室心尖部起搏可以导致心肌复极延长及促进心肌ERK1/2的磷酸化。
목적：탐토우심실심첨부기박대Beagle견심실기전중구급세포외신호조절격매（ERK）1/2린산화수평적영향。방법성년웅성Beagle견12지，년령3~4년，체중20~24kg，수궤분위대조조（n=6）화기박조（n=6），균식입DDD기박기후정공지관폐상태，기박조술후1주시개계기박기병사심실기박최대화。우술전급술후1주、4주관폐기박기후행심전도급초성심동묘기술검사，술후4주시처사동물，관찰심기병리결과，채용면역인적법검측견우심실심기총ERK1/2급기린산화수평적변화。결과량조심실기병리급초성심동묘기술、심솔、QRS시간、총ERK1/2적표체차이균무통계학의의（균P＞0.05）；여대조조상비，술후4주시기박조Tp- e간기、Q- T간기균증대，p- ERK증다（균P＜0.05）。결론우심실심첨부기박가이도치심기복겁연장급촉진심기ERK1/2적린산화。
Objective To investigate the effects of right ventricular apical pacing on electrical remodeling and ERK1/2 phosphorylation in beagle myocardium. Methods 12 male beagles, 3- 4 years old, underwent DDD pacemaker implanta-tion and were randomly divided into two groups (n=6). The pacemakers were turned on and programmed to ensure ventric-ular pacing 1 week after implantation in pacing group and turned off al the time in control group. The ECG and UCG were recorded before, 1 and 4 weeks after procedure with the pacemaker turned off. The beagles were sacrificed 4 weeks after procedure and phosphorylated ERK1 / 2 of right ventricular myocardium was determined by Western blotting. Results There was no statistical difference in the expression of ERK1 / 2, heart rate, QRS duration, BNP, ventricular myocardial pathology and parameters of UCG between the two groups. Tpeak- Tend interval and QT duration were prolonged and the expression of phosphorylated ERK1/2 increased in pacing group compared with control group(P<0.05) 4 weeks after pro-cedure. Conclusion Right ventricular apical pacing may induce myocardial repolarization prolongation and promote ERK1/2 phosphorylation of myocardium.