生态环境学报
生態環境學報
생태배경학보
ECOLOGY AND ENVIRONMENT
2014年
3期
439-443
,共5页
武慧斌%宋正国%沈跃%唐世荣%刘仲齐
武慧斌%宋正國%瀋躍%唐世榮%劉仲齊
무혜빈%송정국%침약%당세영%류중제
微根管%CO2浓度升高%根系形态%水稻
微根管%CO2濃度升高%根繫形態%水稻
미근관%CO2농도승고%근계형태%수도
Minirhizotrons%CO2 treatment%root morphology%rice varieties
Minirhizontrons是一种非破坏性、定点、可直接观测和研究植物根系的新方法。利用微根管Minirhizotrons在试验田的温室大棚内研究CO2浓度升高作用下的水稻根系生长发育，试验采用完全随机处理，探讨CO2浓度升高（800μmol·mol-1）对水稻(Oryza sativa L.)生物量和根系形态的变化差异。结果表明，与CO2对照相比，CO2浓度升高显著增加4个水稻品种(2种杂交籼稻和2种常规籼稻)的地上部生物量，增幅为8.58%~12.66%，平均增加10.61%。CO2浓度升高条件下，根的生物量分别增加了3.16%~12.13%，平均增加8.64%。高CO2浓度对根系形态的影响表明，4种水稻根系对CO2浓度升高都有积极的响应。CO2浓度升高条件下，各根系指标在水稻不同生育期都有显著增加，根长密度、表面积、体积和根数的平均增幅分别为10%~27%、21%~24%、20%~58%和4%~18%。但在水稻生长发育过程中，品种间也存在着差异。CO2浓度升高和对照处理，籼型杂交稻威优644(V644)和金优207(JY207)的根长密度和根数表现出相似的变化趋势；高CO2浓度处理时其根长密度平均都增加了10%，根数平均增加4%和8%。CO2浓度升高和对照处理，2种籼型杂交水稻的根体积和表面积表现出较快的增长幅度，都呈现出近线性的生长趋势；CO2浓度升高处理下其根体积平均增加40%和25%，表面积平均都增加了24%。CO2浓度升高和对照处理，籼型常规稻湘晚12号(XW12)和丰华占(FHZ)的生长变化趋势表现一致，生长发育后期达到一个近似饱和的拐点。CO2浓度升高条件下其根长密度、根数和根体积分别平均增加27%和24%、18%和11%、58%和20%，根表面积平均都增加了21%。
Minirhizontrons是一種非破壞性、定點、可直接觀測和研究植物根繫的新方法。利用微根管Minirhizotrons在試驗田的溫室大棚內研究CO2濃度升高作用下的水稻根繫生長髮育，試驗採用完全隨機處理，探討CO2濃度升高（800μmol·mol-1）對水稻(Oryza sativa L.)生物量和根繫形態的變化差異。結果錶明，與CO2對照相比，CO2濃度升高顯著增加4箇水稻品種(2種雜交秈稻和2種常規秈稻)的地上部生物量，增幅為8.58%~12.66%，平均增加10.61%。CO2濃度升高條件下，根的生物量分彆增加瞭3.16%~12.13%，平均增加8.64%。高CO2濃度對根繫形態的影響錶明，4種水稻根繫對CO2濃度升高都有積極的響應。CO2濃度升高條件下，各根繫指標在水稻不同生育期都有顯著增加，根長密度、錶麵積、體積和根數的平均增幅分彆為10%~27%、21%~24%、20%~58%和4%~18%。但在水稻生長髮育過程中，品種間也存在著差異。CO2濃度升高和對照處理，秈型雜交稻威優644(V644)和金優207(JY207)的根長密度和根數錶現齣相似的變化趨勢；高CO2濃度處理時其根長密度平均都增加瞭10%，根數平均增加4%和8%。CO2濃度升高和對照處理，2種秈型雜交水稻的根體積和錶麵積錶現齣較快的增長幅度，都呈現齣近線性的生長趨勢；CO2濃度升高處理下其根體積平均增加40%和25%，錶麵積平均都增加瞭24%。CO2濃度升高和對照處理，秈型常規稻湘晚12號(XW12)和豐華佔(FHZ)的生長變化趨勢錶現一緻，生長髮育後期達到一箇近似飽和的枴點。CO2濃度升高條件下其根長密度、根數和根體積分彆平均增加27%和24%、18%和11%、58%和20%，根錶麵積平均都增加瞭21%。
Minirhizontrons시일충비파배성、정점、가직접관측화연구식물근계적신방법。이용미근관Minirhizotrons재시험전적온실대붕내연구CO2농도승고작용하적수도근계생장발육，시험채용완전수궤처리，탐토CO2농도승고（800μmol·mol-1）대수도(Oryza sativa L.)생물량화근계형태적변화차이。결과표명，여CO2대조상비，CO2농도승고현저증가4개수도품충(2충잡교선도화2충상규선도)적지상부생물량，증폭위8.58%~12.66%，평균증가10.61%。CO2농도승고조건하，근적생물량분별증가료3.16%~12.13%，평균증가8.64%。고CO2농도대근계형태적영향표명，4충수도근계대CO2농도승고도유적겁적향응。CO2농도승고조건하，각근계지표재수도불동생육기도유현저증가，근장밀도、표면적、체적화근수적평균증폭분별위10%~27%、21%~24%、20%~58%화4%~18%。단재수도생장발육과정중，품충간야존재착차이。CO2농도승고화대조처리，선형잡교도위우644(V644)화금우207(JY207)적근장밀도화근수표현출상사적변화추세；고CO2농도처리시기근장밀도평균도증가료10%，근수평균증가4%화8%。CO2농도승고화대조처리，2충선형잡교수도적근체적화표면적표현출교쾌적증장폭도，도정현출근선성적생장추세；CO2농도승고처리하기근체적평균증가40%화25%，표면적평균도증가료24%。CO2농도승고화대조처리，선형상규도상만12호(XW12)화봉화점(FHZ)적생장변화추세표현일치，생장발육후기체도일개근사포화적괴점。CO2농도승고조건하기근장밀도、근수화근체적분별평균증가27%화24%、18%화11%、58%화20%，근표면적평균도증가료21%。
Mimirhizotrons is a non-destructive, fixed-point, in situ method for directly viewing and studying plant root dynamics. The minirhizontrons for studying biomass and root morphology variation of rice (Oryza sativa L.) in the field of greenhouse were employed in the study. Completely randomized design was used. The results showed that, the aboveground biomass of four rice varieties (two indica hybid and two indica convention) increased significantly from 8.58% to 12.66% (mean value 8.64%) with elevating CO2 concentration. There were positive effects on root length density, surface area, volume, and tips number at four growth stages (tillering, heading, grain filling period, and ripening period) of rice varieties with elevated atmospheric CO2. The ranges of root length density, surface area, volume, and tips number were 10%-27%, 21%-24%, 20%-58%, and 4%-18%, respectively. There were significant differences among rice varieties during rice root growth with CO2 treatment. The root length density and tips of indica hybrid rice varieties (V644 and JY207) showed the similar trends in the rice growth periods, the mean percentages of root length density and tips increased by 10%, 10%, and 4%, 8%, respectively. The average values of root volume and surface area of V644 and JY207 were up to 40%, 25%, and 24%, 24%, respectively, indicating linear growth trend. The indica convention rice XW12 and FHZ had the same growth trend, while the increase rate of root growth parameters was relatively gentle and reached an approximation saturation point at the late growth stage of rice. Under CO2 treatment condition, the four root parameters (root length density, tips, volume, and surface area) of XW12 increased 27%, 18%, 58%, and 21%, ,and of FHZ were up to 24%, 11%, 20%, and 21%, respectively.
