CAJ | 학술논문

1398食品级蛋白酶是一种由枯草芽胞杆菌1398发酵产生的用于食品加工处理的中性蛋白酶。目前国内生产工艺制取的固体酶制剂存在菌体未分离、产品颜色深、酸臭味大等缺点。本文以开发无色无臭的液体蛋白酶制剂为目的，对蛋白酶的发酵后提取、纯化工艺等进行了深入研究，分别考察了离子交换树脂层析和工业级大孔树脂对发酵液中1398中性蛋白酶的分离纯化效果，对纯化后的蛋白酶进行了Maldi-Tof质谱分析，并对编码该蛋白酶的基因进行了测序和序列分析，为进一步利用基因工程重组技术生产该蛋白酶奠定了研究基础。
1398식품급단백매시일충유고초아포간균1398발효산생적용우식품가공처리적중성단백매。목전국내생산공예제취적고체매제제존재균체미분리、산품안색심、산취미대등결점。본문이개발무색무취적액체단백매제제위목적，대단백매적발효후제취、순화공예등진행료심입연구，분별고찰료리자교환수지층석화공업급대공수지대발효액중1398중성단백매적분리순화효과，대순화후적단백매진행료Maldi-Tof질보분석，병대편마해단백매적기인진행료측서화서렬분석，위진일보이용기인공정중조기술생산해단백매전정료연구기출。
The food-grade 1398 neutral protease ( 1398 NprE) , produced through fermentation using Bacillus subtilis AS1398, is widely used in food processing industry. However, the quality of present 1398 NprE is rather disfavored, brought by its dark color, unpleasant odors or contamination problem. With the purpose of development of 1398 NprE liquid products that better meets strict standard of food processing industry, we attempted to purify the protease using sepharose FF column and macroporous resin chromatography, and analysed the target protein with Maldi-Tof spectrometry; DNA and peptide sequences of 1398 NprE were further revealed and characterized by multiple alignment analysis, which laid a solid ground for further studies of strain development for production of better recombinant neutral proteases through genetic engineering biotechnologies.