华南师范大学学报（自然科学版）
華南師範大學學報（自然科學版）
화남사범대학학보（자연과학판）
JOURNAL OF SOUTH CHINA NORMAL UNIVERSITY （NATURAL SCIENCE EDITION）
2014年
3期
112-117
,共6页
石斛%显微鉴定%红外光谱鉴定%AFLP
石斛%顯微鑒定%紅外光譜鑒定%AFLP
석곡%현미감정%홍외광보감정%AFLP
medicinal Dendrobii%microscopical identification%infrared spectroscopy(IR)identification%AFLP
采用徒手切片和显微测量法观察分析铁皮石斛、细茎石斛和齿辨石斛3种药用石斛的显微特征；用傅里叶红外光谱（FT-IR）及其导数光谱法测定其红外光谱特征；用 AFLP 分子标记方法分析其分子鉴别特征.结果表明，3种药用石斛显微特征存在一定差异，铁皮石斛的角质层比较薄，厚度约4.9μm；3种药用石斛茎表皮细胞为不均匀加厚型、无增厚型和均匀加厚型，齿瓣石斛的厚壁组织细胞壁加厚程度明显，厚度为30.0μm.在3种药用石斛二阶导数光谱特征中，1585、1217和1049 cm -1是细茎石斛的特有峰，1569、1450、1225和1073 cm -1是铁皮石斛和齿瓣石斛的共有特征峰，1011 cm -1是细茎石斛和齿瓣石斛的共有特征峰. AFLP 扩增和聚类分析结果表明，3种药用石斛的总扩增位点为113个，其中多态性扩增位点共111个，铁皮石斛特异性扩增位点明显多于细茎石斛和齿瓣石斛.显微结构观察、红外光谱和 AFLP 分子鉴定方法的综合应用对3种药用石斛提供了更准确的鉴别信息.
採用徒手切片和顯微測量法觀察分析鐵皮石斛、細莖石斛和齒辨石斛3種藥用石斛的顯微特徵；用傅裏葉紅外光譜（FT-IR）及其導數光譜法測定其紅外光譜特徵；用 AFLP 分子標記方法分析其分子鑒彆特徵.結果錶明，3種藥用石斛顯微特徵存在一定差異，鐵皮石斛的角質層比較薄，厚度約4.9μm；3種藥用石斛莖錶皮細胞為不均勻加厚型、無增厚型和均勻加厚型，齒瓣石斛的厚壁組織細胞壁加厚程度明顯，厚度為30.0μm.在3種藥用石斛二階導數光譜特徵中，1585、1217和1049 cm -1是細莖石斛的特有峰，1569、1450、1225和1073 cm -1是鐵皮石斛和齒瓣石斛的共有特徵峰，1011 cm -1是細莖石斛和齒瓣石斛的共有特徵峰. AFLP 擴增和聚類分析結果錶明，3種藥用石斛的總擴增位點為113箇，其中多態性擴增位點共111箇，鐵皮石斛特異性擴增位點明顯多于細莖石斛和齒瓣石斛.顯微結構觀察、紅外光譜和 AFLP 分子鑒定方法的綜閤應用對3種藥用石斛提供瞭更準確的鑒彆信息.
채용도수절편화현미측량법관찰분석철피석곡、세경석곡화치변석곡3충약용석곡적현미특정；용부리협홍외광보（FT-IR）급기도수광보법측정기홍외광보특정；용 AFLP 분자표기방법분석기분자감별특정.결과표명，3충약용석곡현미특정존재일정차이，철피석곡적각질층비교박，후도약4.9μm；3충약용석곡경표피세포위불균균가후형、무증후형화균균가후형，치판석곡적후벽조직세포벽가후정도명현，후도위30.0μm.재3충약용석곡이계도수광보특정중，1585、1217화1049 cm -1시세경석곡적특유봉，1569、1450、1225화1073 cm -1시철피석곡화치판석곡적공유특정봉，1011 cm -1시세경석곡화치판석곡적공유특정봉. AFLP 확증화취류분석결과표명，3충약용석곡적총확증위점위113개，기중다태성확증위점공111개，철피석곡특이성확증위점명현다우세경석곡화치판석곡.현미결구관찰、홍외광보화 AFLP 분자감정방법적종합응용대3충약용석곡제공료경준학적감별신식.
The freehand section and micrometer analysis were conducted to study the microscopic characters of the stems of Dendrobium officinale,D. moniliforme and D. devonianumt. The IR characters of three medicinal Dendrobii were analyzed by FT-IR and the second order derivative FT-IR. The molecular characters of the three medicinal Dendrobii were amplified and sequenced by AFLP. Results showed that the cell wall thickness of cuticula of D. offi-cinale was 4. 9 μm that was fairly thinner than the other two species. The cell wall thickness of stem epidermis in D. officinale is 9. 1 μm,with non uniform thickness,D. moniliforme has non thickened,and D. devonianum has uniform thickened. The characteristic peaks in the second order derivative FT-IR for D. moniliforme are 1 585, 1 217 and 1 049 cm - 1 ,D. officinale and D. devonianum have special peaks of 1 569,1 450,1 225 and 1 073 cm - 1 ,D. moniliforme and D. devonianum had the special peak of 1 011 cm - 1 . The three medicinal Dendrobii gen-erated 113 amplified loci,111 of which was polymorphic loci which accounted for 99. 7% ,and the special loci number of D. officinale was obviously more than other two species in the total amplified loci by AFLP. The three medicinal Dendrobii can be distinguished by the comprehensive application of the microscopic,IR and AFLP char-acteristics.
