黑龙江医药
黑龍江醫藥
흑룡강의약
HEILONGJIANG MEDICAL JOURNAL
2014年
3期
522-523,524
,共3页
足细胞%α-actinin-4%白蛋白
足細胞%α-actinin-4%白蛋白
족세포%α-actinin-4%백단백
podocytes%α-actinin-4%BSA
目的：观察足细胞损伤及药物干预后细胞骨架蛋白mRNA的变化，以进一步研究足细胞病的发病机制。方法：体外培养条件永生化小鼠足细胞，设立分组：对照组、足细胞损伤组（BSA 20mg/ml）、地塞米松组（地塞米松0.1umol/L+白蛋白20mg/ml），分别于12h、24h、48h检测足细胞内α-actinin4 mRNA表达变化。结果：足细胞损伤组足细胞内α-actinin4 mRNA水平随时间变化逐渐降低，地塞米松组先降低后升高。结论：地塞米松可以改善足细胞损伤后骨架蛋白α-actinin-4的mRNA表达的变化。
目的：觀察足細胞損傷及藥物榦預後細胞骨架蛋白mRNA的變化，以進一步研究足細胞病的髮病機製。方法：體外培養條件永生化小鼠足細胞，設立分組：對照組、足細胞損傷組（BSA 20mg/ml）、地塞米鬆組（地塞米鬆0.1umol/L+白蛋白20mg/ml），分彆于12h、24h、48h檢測足細胞內α-actinin4 mRNA錶達變化。結果：足細胞損傷組足細胞內α-actinin4 mRNA水平隨時間變化逐漸降低，地塞米鬆組先降低後升高。結論：地塞米鬆可以改善足細胞損傷後骨架蛋白α-actinin-4的mRNA錶達的變化。
목적：관찰족세포손상급약물간예후세포골가단백mRNA적변화，이진일보연구족세포병적발병궤제。방법：체외배양조건영생화소서족세포，설립분조：대조조、족세포손상조（BSA 20mg/ml）、지새미송조（지새미송0.1umol/L+백단백20mg/ml），분별우12h、24h、48h검측족세포내α-actinin4 mRNA표체변화。결과：족세포손상조족세포내α-actinin4 mRNA수평수시간변화축점강저，지새미송조선강저후승고。결론：지새미송가이개선족세포손상후골가단백α-actinin-4적mRNA표체적변화。
Objective:To observe the change of the mRNA of cytoskeletal protein in the podocyte cell after injured and medicine, and to explore the possible mechanism of podocyte injury. Methods:Mouse podocytes were cultured in vitro.Podocytes were divided into three groups: control, BSA overload(20mg/ml) and dexamethasone (BSA20 mg/ml and dexamethasone 0.1umol/L). PT-PCR was used to detect the levels ofα-actinin-4 mRNA when 12h, 24h and 48h. Results:The expression level was decreasd steadily with the change of time in BSA overload . Otherwise in dexamethasone, the expression level ofα-actinin4 mRNA appeared to have a decline trend initialiy ,then apppear an ascent with the change over time. Conclusion:Dexamethasone could change the levels of mRNA after podocytes damaged.
