黑龙江医药
黑龍江醫藥
흑룡강의약
HEILONGJIANG MEDICAL JOURNAL
2014年
3期
500-501,502
,共3页
脑安片%人参皂苷Re%高效液相色谱法%含量测定
腦安片%人參皂苷Re%高效液相色譜法%含量測定
뇌안편%인삼조감Re%고효액상색보법%함량측정
Naoan tablets%Ginsenoside Re%RP-HPLC%determination
目的：建立脑安片中人参皂苷Re的含量测定方法。方法：提取供试品，以高效液相色谱法测定人参皂苷Re的含量，流动相采用乙腈和水进行梯度洗脱，流速1 ml/min，柱温35℃，检测波长203 nm。结果：人参皂苷Re在96.96～484.8 ng/μl范围内呈良好的线性关系，回归方程为Y=3.72651X+0.73355，r=0.99999；平均回收率为99.5%（RSD 0.72%），重复性RSD为1.28%。结论：该方法准确、快捷、重复性好。
目的：建立腦安片中人參皂苷Re的含量測定方法。方法：提取供試品，以高效液相色譜法測定人參皂苷Re的含量，流動相採用乙腈和水進行梯度洗脫，流速1 ml/min，柱溫35℃，檢測波長203 nm。結果：人參皂苷Re在96.96～484.8 ng/μl範圍內呈良好的線性關繫，迴歸方程為Y=3.72651X+0.73355，r=0.99999；平均迴收率為99.5%（RSD 0.72%），重複性RSD為1.28%。結論：該方法準確、快捷、重複性好。
목적：건립뇌안편중인삼조감Re적함량측정방법。방법：제취공시품，이고효액상색보법측정인삼조감Re적함량，류동상채용을정화수진행제도세탈，류속1 ml/min，주온35℃，검측파장203 nm。결과：인삼조감Re재96.96～484.8 ng/μl범위내정량호적선성관계，회귀방정위Y=3.72651X+0.73355，r=0.99999；평균회수솔위99.5%（RSD 0.72%），중복성RSD위1.28%。결론：해방법준학、쾌첩、중복성호。
Objective: To develop a method for the determination of Ginsenoside Re in Naoan tablet. Methods: An RP-HPLC method of the determination ginsengside Re content was applied with the UV wavelength seted at 203 nm.The mobile phase consisted of solution A (acetonitrile)and solution B(water) with gradient elute.The flow rate was 1 ml/min and the column temperature was 35℃. Results:The calibration curve was in good linearity over the range of 96.96~484.8 ng/μl, the regression equation is Y=3.72651X+0.73355,r=0.99999;The average returns-ratio is99.5%(RSD 0.72%), duplicated RSD is 1.28%. Conclusion:The method is accu-rate, quick,the duplication is good.
