CAJ | 학술논문

目的：本文是论述测定广西荨麻中总皂苷的含量的方法。方法：以胡萝卜苷为标准对照品，5%香草醛-冰醋酸、高氯酸为显色剂，采用紫外分光光度法，在542nm处测定吸光度，计算荨麻中总皂苷的含量。结果：荨麻的总皂苷浓度在0.0018-0.0072mg/ml与吸光度呈良好的线性关系Y=11.528X+0.117（r=0.9988），平均回收率为98.69%（n=6），RSD=1.11%；荨麻中总皂苷的含量为21.3mg/g。并建立了荨麻中总皂苷超声提取的提取方法和紫外-可见分光光度法对其含量测定方法。结论：此方法方便，准确性高，稳定性好，可用于荨麻药材的总皂苷的含量测定。
목적：본문시논술측정엄서담마중총조감적함량적방법。방법：이호라복감위표준대조품，5%향초철-빙작산、고록산위현색제，채용자외분광광도법，재542nm처측정흡광도，계산담마중총조감적함량。결과：담마적총조감농도재0.0018-0.0072mg/ml여흡광도정량호적선성관계Y=11.528X+0.117（r=0.9988），평균회수솔위98.69%（n=6），RSD=1.11%；담마중총조감적함량위21.3mg/g。병건립료담마중총조감초성제취적제취방법화자외-가견분광광도법대기함량측정방법。결론：차방법방편，준학성고，은정성호，가용우담마약재적총조감적함량측정。
Objective:The paper establishs the abstract and determination methods of total saponins from nettle. The carrot sapo-nins was selected as comparison product and 5%vanillinacetic acid-HCLO solution was selected as color-producing reagent. I deter-mine absorbability in the point of 542nm by spectrophotometry method and calculate the total saponins in nettle. The calibration curve was linear over the range of 0.0018-0.0072mg/ml with the correlation of 0. 9988, Y=11.528X+0.117 , the average recoveries of di-oscin was 98.69%( n=6) ,RSD=1.11%. The context of the carrot saponins in nettle is 21.3mg/g. The method of nettle of ultrasonic extraction of total saponin is simple accurate. The mehod is good stability. It can be used for quality control of medicinal nettle.