CAJ | 학술논문

目的：研究经环氧乙烷灭菌的培养皿对Ames试验的影响。方法：采用Ames试验平板掺入法，分别使用不同储存时间及是否通风等情况下的环氧乙烷灭菌培养皿，计数TA97、TA98、TA100、TA102及TA1535菌株的回变菌落数。结果：灭菌后存放1个月左右(25 d)的培养皿中环氧乙烷的残留在活化和非活化两种条件下均能引起TA100和TA1535菌株的回变菌落数增多(TA100，约自发回变数的1.5倍；TA1535，约自发回变数的10倍)，而经通风或延长培养皿储存时间可减少环氧乙烷残留并消除这种致突变作用。结论：在一定条件下，经环氧乙烷灭菌的培养皿对Ames试验中TA100、TA1535菌株有致突变性。
목적：연구경배양을완멸균적배양명대Ames시험적영향。방법：채용Ames시험평판참입법，분별사용불동저존시간급시부통풍등정황하적배양을완멸균배양명，계수TA97、TA98、TA100、TA102급TA1535균주적회변균락수。결과：멸균후존방1개월좌우(25 d)적배양명중배양을완적잔류재활화화비활화량충조건하균능인기TA100화TA1535균주적회변균락수증다(TA100，약자발회변수적1.5배；TA1535，약자발회변수적10배)，이경통풍혹연장배양명저존시간가감소배양을완잔류병소제저충치돌변작용。결론：재일정조건하，경배양을완멸균적배양명대Ames시험중TA100、TA1535균주유치돌변성。
OBJECTIVE: Study on effects of the ethylene oxide-sterilized Petri plate on Ames test. METHODS:In the plate incorporation test of Salmonella typhimurium,the revertant colonies of TA97, TA98,TA100,TA102 and TA1535 on the ethylene oxide sterilized Petri plates stored for different periods of time or by ventilation was counted. RESULTS:Ethylene oxide residuals in the Petri plates one month after sterilized increased the revertant colonies of TA100 and TA1535(TA100,about 1.5 fold of the spontaneous revertant colonies;TA1535,about 10 fold of the spontaneous revertant colonies),and extending storage or ventilation could reduce the plate ethylene oxide residuals to a level that caused no mutagenetic effects. CONCLUSION:Under certain conditions,ethylene oxide-sterilized Petri plate could induce TA100 and TA1535 mutagenicity on Ames test.