癌变·畸变·突变
癌變·畸變·突變
암변·기변·돌변
CARCINOGENSES,TERATOGENSIS AND MUTAGENESIS
2014年
3期
221-224
,共4页
藏茵陈总萜酮%Ames实验%微核实验%致突变%抗突变
藏茵陳總萜酮%Ames實驗%微覈實驗%緻突變%抗突變
장인진총첩동%Ames실험%미핵실험%치돌변%항돌변
total terpene ketones from Swertia mussotii%Ames test%micronucleus test%mutagenicity%anti-mutagenicity
目的：研究藏茵陈总萜酮的致突变及抗突变作用。方法：致突变实验采用Ames实验及小鼠体内微核实验。Ames实验采用常规掺入法；微核实验采用连续灌胃给药10 d的骨髓嗜多染红细胞微核计数法。抗突变实验采用体内、体外两种方法，体外法选用TA100及TA102菌株与环磷酰胺(每皿200μg)和丝裂霉素C(每皿2 g)共孵育30 min后，分别给予藏茵陈总萜酮每皿156、312、μ625、1250及2500μg，检测其对阳性剂诱发的已突变菌落的保护作用；体内实验采用藏茵陈总萜酮25、50及100 mg/kg小鼠连续灌胃给药10 d后，给予40 mg/kg环磷酰胺或2 mg/kg丝裂霉素C，计算微核率，检测其对未发生突变的骨髓细胞的保护作用。结果：致突变实验中，藏茵陈总萜酮在每皿<2500μg剂量下，未诱发Ames实验各菌株回变菌落数增加；在每皿<40 mg/kg剂量下，未诱发骨髓嗜多染红细胞微核率增高。抗突变实验中，藏茵陈总萜酮在每皿625~2500μg范围内，可使阳性致突变剂环磷酰胺和丝裂霉素C所诱发的高回变菌落数出现明显降低；藏茵陈总萜酮在50~100 mg/kg剂量范围，可使阳性剂环磷酰胺或丝裂霉素C所诱发的高微核率出现明显降低。结论：本实验条件下，藏茵陈总萜酮未表现出诱导基因突变和染色体畸变作用，且有显著的抗突变作用。
目的：研究藏茵陳總萜酮的緻突變及抗突變作用。方法：緻突變實驗採用Ames實驗及小鼠體內微覈實驗。Ames實驗採用常規摻入法；微覈實驗採用連續灌胃給藥10 d的骨髓嗜多染紅細胞微覈計數法。抗突變實驗採用體內、體外兩種方法，體外法選用TA100及TA102菌株與環燐酰胺(每皿200μg)和絲裂黴素C(每皿2 g)共孵育30 min後，分彆給予藏茵陳總萜酮每皿156、312、μ625、1250及2500μg，檢測其對暘性劑誘髮的已突變菌落的保護作用；體內實驗採用藏茵陳總萜酮25、50及100 mg/kg小鼠連續灌胃給藥10 d後，給予40 mg/kg環燐酰胺或2 mg/kg絲裂黴素C，計算微覈率，檢測其對未髮生突變的骨髓細胞的保護作用。結果：緻突變實驗中，藏茵陳總萜酮在每皿<2500μg劑量下，未誘髮Ames實驗各菌株迴變菌落數增加；在每皿<40 mg/kg劑量下，未誘髮骨髓嗜多染紅細胞微覈率增高。抗突變實驗中，藏茵陳總萜酮在每皿625~2500μg範圍內，可使暘性緻突變劑環燐酰胺和絲裂黴素C所誘髮的高迴變菌落數齣現明顯降低；藏茵陳總萜酮在50~100 mg/kg劑量範圍，可使暘性劑環燐酰胺或絲裂黴素C所誘髮的高微覈率齣現明顯降低。結論：本實驗條件下，藏茵陳總萜酮未錶現齣誘導基因突變和染色體畸變作用，且有顯著的抗突變作用。
목적：연구장인진총첩동적치돌변급항돌변작용。방법：치돌변실험채용Ames실험급소서체내미핵실험。Ames실험채용상규참입법；미핵실험채용련속관위급약10 d적골수기다염홍세포미핵계수법。항돌변실험채용체내、체외량충방법，체외법선용TA100급TA102균주여배린선알(매명200μg)화사렬매소C(매명2 g)공부육30 min후，분별급여장인진총첩동매명156、312、μ625、1250급2500μg，검측기대양성제유발적이돌변균락적보호작용；체내실험채용장인진총첩동25、50급100 mg/kg소서련속관위급약10 d후，급여40 mg/kg배린선알혹2 mg/kg사렬매소C，계산미핵솔，검측기대미발생돌변적골수세포적보호작용。결과：치돌변실험중，장인진총첩동재매명<2500μg제량하，미유발Ames실험각균주회변균락수증가；재매명<40 mg/kg제량하，미유발골수기다염홍세포미핵솔증고。항돌변실험중，장인진총첩동재매명625~2500μg범위내，가사양성치돌변제배린선알화사렬매소C소유발적고회변균락수출현명현강저；장인진총첩동재50~100 mg/kg제량범위，가사양성제배린선알혹사렬매소C소유발적고미핵솔출현명현강저。결론：본실험조건하，장인진총첩동미표현출유도기인돌변화염색체기변작용，차유현저적항돌변작용。
OBJECTIVE:To study the mutagenic and anti-mutagenic activities of total terpene ketones from Swertia mussotii (TTKS) at different doses. METHODS:Mutagenic experiments:conventional Ames test and micronucleus test were selected. Ames experiment was used with the incorporation method. Micronucleus test was conducted in mice which were treated orally once a day for 10 days consecutively. Anti-mutagenic experiments:methods in vitro,pre-incubated the TA100 and TA102 strains with CP (200 μg/plate) or MMC (2 μg/plate) for 30 minutes,then mixed the above materials with TTKS ( 156,312,625,1 250 and 2 500 μg/plate ),so as to detect the protective effects of TTKS on mutational colonies induced by positive agent. Mice were exposed to CP (40 mg/kg) or MMC (2 mg/kg) after continuous irrigation of TTKS (25, 50 and 100 mg/kg ) for 10 days,the micronucleus rate was calculated in order to detect the protective effects of TTKS on non-mutation bone marrow cells. RESULTS:In mutagenic test,TTKS at a dose lower than 2 000 μg/plate induced no obvious increase in mutagenicity of the four strains. TTKS at a dose lower than 40 mg/kg did not increase the micronucleus frequencies of polychromatic erythrocytes. In anti-mutagenic test,comparing with the positive groups of CP and MMC,TTKS at 625-2 500 μg/plate could decrease the colony numbers in T100 and T102 strains. TTKS at 50-100 mg/kg could significantly reduce the rate of micronucleus of polychromatic erythrocytes in mice bone marrow induced by CP and MMC as compared with the positive control. CONCLUSION:Under these experimental conditions,TTKS showed no effect of mutagenesis and could confer significant anti-mutagenic protection.
