CAJ | 학술논문

目的：探讨血浆中载脂蛋白A (apoAⅠ)含量和哺乳动物雷帕霉素靶蛋白(mTOR)活性在维吾尔族妇女宫颈病变进程中的动态变化及评价其对于宫颈癌预警的灵敏度、特异性和准确性。方法：收集新疆地区维吾尔族宫颈病变血浆样本共82例(宫颈炎22例，CINⅡ~Ⅲ20例，宫颈癌早期20例，宫颈癌晚期20例)，用酶联免疫法检测血浆中apoAⅠ蛋白含量和mTOR活性，分析其与宫颈病变进程的关系及相关性，评价其对于宫颈癌预警的灵敏度、特异性和准确性。结果：随着宫颈炎到CINⅡ~Ⅲ、宫颈癌早期和晚期发病过程，血浆apoAⅠ含量明显降低而mTOR活性上升，与正常对照宫颈炎组比较，其差异均有统计学意义(P＜0.05)；apoAⅠ的表达与mTOR活性呈低度负相关(r＜0.4，＜0.05)；血浆mTOR活性的预警灵敏度、特异度和准确度较高，分别为94.0%、60.0%、80.0%，而apoAⅠ分别为70.1%、60.0%、29.0%。从不同指标联合检测的角度分析，apoAⅠ和mTOR联合检测的灵敏度、特异度和准确度分别为82.1%、60.0%、54.5%。结论：维吾尔族妇女宫颈癌发生发展与血浆中apoAⅠ含量和mTOR活性动态变化密切相关，可以成为宫颈癌早期诊断的预警或疗效评估的重要指标。ⅠP
목적：탐토혈장중재지단백A (apoAⅠ)함량화포유동물뢰파매소파단백(mTOR)활성재유오이족부녀궁경병변진정중적동태변화급평개기대우궁경암예경적령민도、특이성화준학성。방법：수집신강지구유오이족궁경병변혈장양본공82례(궁경염22례，CINⅡ~Ⅲ20례，궁경암조기20례，궁경암만기20례)，용매련면역법검측혈장중apoAⅠ단백함량화mTOR활성，분석기여궁경병변진정적관계급상관성，평개기대우궁경암예경적령민도、특이성화준학성。결과：수착궁경염도CINⅡ~Ⅲ、궁경암조기화만기발병과정，혈장apoAⅠ함량명현강저이mTOR활성상승，여정상대조궁경염조비교，기차이균유통계학의의(P＜0.05)；apoAⅠ적표체여mTOR활성정저도부상관(r＜0.4，＜0.05)；혈장mTOR활성적예경령민도、특이도화준학도교고，분별위94.0%、60.0%、80.0%，이apoAⅠ분별위70.1%、60.0%、29.0%。종불동지표연합검측적각도분석，apoAⅠ화mTOR연합검측적령민도、특이도화준학도분별위82.1%、60.0%、54.5%。결론：유오이족부녀궁경암발생발전여혈장중apoAⅠ함량화mTOR활성동태변화밀절상관，가이성위궁경암조기진단적예경혹료효평고적중요지표。ⅠP
OBJECTIVE:To explore the relationships of the dynamic changes of plasma apoAⅠcontent and mTOR activity with cervical carcinogenesis development in Uyghur women. METHODS:We collected 82 plasma samples from Uyghur women with cervicitis (CV) (22 cases),cervical intraepithelial neoplasia (CIN) Ⅱ-Ⅲ (20 cases),early stage (Ⅰ-Ⅱ) (20 cases) and advanced stage (ⅡB-Ⅳ) (20 cases) cervical squamous cell carcinoma, quantitatively determined the content of plasma proteins apoAⅠand the activity of mTOR by enzyme linked immunosorbent assay (ELISA) and statistical analysis,in order to analyze the correlation with the progression of cervical lesion and evaluate the accuracy,specificity and sensitivity of candidate plasma protein markers for early warning of cervical cancer. RESULTS:With the development of cervicitis to CINⅡ-Ⅲ,early stage or advanced stage cervical cancer,the changes of two plasma proteins altered gradually,with obvious decrease in the content of apoAⅠ,and increase in the activity of mTOR,with statistical differences for all (P<0.05). Correlation analysis of cervical cancerspecific protein indicators found that mTOR and apoAⅠshowed a low negative correlation (r<0.4,P<0.05). The sensitivity,specificity,and accuracy of detection,for early warning of cervical cancer of the mTOR protein were 94.0%, 60.0% and 80.0%,respectively;and for apoAⅠwere 70.1%, 60.0% and 29.0%,respectively. apoAⅠ/mTOR combined detection of sensitivity,specificity, and accuracy were 82.1%, 60.0% and 54.5%,respectively. CONCLUSION:The dynamic changes of apoAⅠcontent and mTOR activity were important indicators of the procession of cervical lesions,and expected to assist with the early diagnosis of cervical cancer.