CAJ | 학술논문

目的：研究纳米二氧化钛(TiO2)对大鼠NK细胞活性和相关细胞因子的影响。方法：以SD大鼠为研究模对象，随机分为0.5、4和32 mg/kg 3个纳米TiO2染毒组，对照组(生理盐水)和微米TiO2染毒组(32 mg/kg)。采用支气管滴注染毒，每周2次，实验持续28 d。分别采用流式细胞仪分析外周血NK细胞数目，乳酸脱氢酶释放法检测脾脏NK细胞活性，液相芯片技术测定血清细胞因子IL-2和IFN-γ含量。结果：随着纳米TiO2染毒剂量的增加，脾脏NK细胞活性逐渐增加，高剂量组NK细胞活性与对照组和微米TiO2组相比，差别均具有统计学意义(P<0.05)。微米TiO2组与对照组相比，脾脏NK细胞活性无明显变化(P>0.05)。各剂量组外周血NK细胞数目及血清细胞因子IL-2、IFN-γ的变化，与对照组相比差别均无统计学意义(>0.05)。结论：在本实验剂量范围内，纳米TiO2可刺激机体产生免疫应答，增加脾脏NK细胞活性，但NK细胞活性及相关细胞因子的长期变化及潜在的机制仍需要进一步探讨。P
목적：연구납미이양화태(TiO2)대대서NK세포활성화상관세포인자적영향。방법：이SD대서위연구모대상，수궤분위0.5、4화32 mg/kg 3개납미TiO2염독조，대조조(생리염수)화미미TiO2염독조(32 mg/kg)。채용지기관적주염독，매주2차，실험지속28 d。분별채용류식세포의분석외주혈NK세포수목，유산탈경매석방법검측비장NK세포활성，액상심편기술측정혈청세포인자IL-2화IFN-γ함량。결과：수착납미TiO2염독제량적증가，비장NK세포활성축점증가，고제량조NK세포활성여대조조화미미TiO2조상비，차별균구유통계학의의(P<0.05)。미미TiO2조여대조조상비，비장NK세포활성무명현변화(P>0.05)。각제량조외주혈NK세포수목급혈청세포인자IL-2、IFN-γ적변화，여대조조상비차별균무통계학의의(>0.05)。결론：재본실험제량범위내，납미TiO2가자격궤체산생면역응답，증가비장NK세포활성，단NK세포활성급상관세포인자적장기변화급잠재적궤제잉수요진일보탐토。P
OBJECTIVE:To explore the effects of titanium dioxide (TiO2) nanoparticles on Natural Killer (NK) cell activity and related cytokines in rats. METHODS:Sprague Dawley rats were randomly divided into 0.5,4 and 32 mg/kg TiO2 nanoparticles exposure groups,control group (0.9%sodium chloride solution),and TiO2 micro-sized particles exposure group (32 mg/kg). Rats were treated by intratracheal instillation of these substances in suspension twice a week,a total of 28 consecutive days. NK cell population in peripheral blood was measured by flow cytometry. NK cell killing activity in the spleen was analyzed by lactate dehydrogenase (LDH) release assay. Cytokines IL-2 and INF-γwere measured by liquid phase chips method. RESULTS:The NK cell activity increased with the rise of TiO2 exposure dose. Significant difference in NK cell activity in the spleen was found in the group exposed to 32 mg/kg TiO2 nanoparticles compared with the control group (P<0.05). No significant difference was found between control group and micro-sized TiO2 group (P>0.05). Compared with micro-sized TiO2 group,NK cell activity was found to be significantly increased in 32 mg/kg TiO2 nanoparticles exposure group (P<0.05). No significant change of NK cell populations,IL-2 or INF-γwas observed compared with control group and micro-sized group (P>0.05). CONCLUSION:TiO2 nanoparticles could trigger systemic immune responses,and enhance NK cell killing activity in the spleen in the studied dosage range. However,further research is needed to confirm the long-term changes in NK cell populations,NK cell killing activity and related cytokines,as well as the potential mechanisms.